Detection of high frequency of MAD20 allelic variants of Plasmodium falciparum merozoite surface protein 1 gene from Adama and its surroundings, Oromia, Ethiopia.
Genetic polymorphism
Msp-1
Multiplicity of infection
P. falciparum
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
27 Sep 2021
27 Sep 2021
Historique:
received:
10
06
2021
accepted:
09
09
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
24
11
2021
Statut:
epublish
Résumé
One of the major challenges in developing an effective vaccine against asexual stages of Plasmodium falciparum is genetic polymorphism within parasite population. Understanding the genetic polymorphism like block 2 region of merozoite surface protein-1 (msp-1) gene of P. falciparum enlighten mechanisms underlining disease pathology, identification of the parasite clone profile from the isolates, transmission intensity and potential deficiencies of the ongoing malaria control and elimination efforts in the locality. Detailed understanding of local genetic polymorphism is an input to pave the way for better management, control and elimination of malaria. The aim of this study was to detect the most frequent allelic variant of the msp-1 gene of P. falciparum clinical isolates from selected health facilities in Adama town and its surroundings, Oromia, Ethiopia. One hundred thirty-nine clinical isolates were successfully amplified for msp-1 gene using specific primers. Nested PCR amplification was conducted targeting K1, MAD20, and R033 alleles followed by gel electrophoresis for fragment analysis. Based on the detection of a PCR fragment, infections were classified as monoclonal or multiple infections. 19 different size polymorphism of msp-1 gene were identified in the study, with 67(48%) MAD20, 18 (13%) K-1 and 18 (13%) RO33 allelic family. Whereas, the multiple infections were 21(15%), 8 (5.8%), 4(2.9%), 3(2.2%) for MAD20 + K-1, MAD20 + RO33, K-1 + RO33, and MAD20 + K-1, RO33, respectively. The overall Multiplicity of infection (MOI) was 1.3 and the expected heterozygosity (He) was 0.39 indicating slightly low falciparum malaria transmission. The status of msp-1 allele size polymorphism, MOI and He observed in the study revealed the presence of slightly low genetic diversity of P. falciparum clinical isolates. However, highly frequent MAD20 allelic variant was detected from clinical isolates in the study area. Moreover, the driving force that led to high predominance of MAD20 allelic variant revealed in such malaria declining region demands further research.
Sections du résumé
BACKGROUND
BACKGROUND
One of the major challenges in developing an effective vaccine against asexual stages of Plasmodium falciparum is genetic polymorphism within parasite population. Understanding the genetic polymorphism like block 2 region of merozoite surface protein-1 (msp-1) gene of P. falciparum enlighten mechanisms underlining disease pathology, identification of the parasite clone profile from the isolates, transmission intensity and potential deficiencies of the ongoing malaria control and elimination efforts in the locality. Detailed understanding of local genetic polymorphism is an input to pave the way for better management, control and elimination of malaria. The aim of this study was to detect the most frequent allelic variant of the msp-1 gene of P. falciparum clinical isolates from selected health facilities in Adama town and its surroundings, Oromia, Ethiopia.
METHODS
METHODS
One hundred thirty-nine clinical isolates were successfully amplified for msp-1 gene using specific primers. Nested PCR amplification was conducted targeting K1, MAD20, and R033 alleles followed by gel electrophoresis for fragment analysis. Based on the detection of a PCR fragment, infections were classified as monoclonal or multiple infections.
RESULTS
RESULTS
19 different size polymorphism of msp-1 gene were identified in the study, with 67(48%) MAD20, 18 (13%) K-1 and 18 (13%) RO33 allelic family. Whereas, the multiple infections were 21(15%), 8 (5.8%), 4(2.9%), 3(2.2%) for MAD20 + K-1, MAD20 + RO33, K-1 + RO33, and MAD20 + K-1, RO33, respectively. The overall Multiplicity of infection (MOI) was 1.3 and the expected heterozygosity (He) was 0.39 indicating slightly low falciparum malaria transmission.
CONCLUSION
CONCLUSIONS
The status of msp-1 allele size polymorphism, MOI and He observed in the study revealed the presence of slightly low genetic diversity of P. falciparum clinical isolates. However, highly frequent MAD20 allelic variant was detected from clinical isolates in the study area. Moreover, the driving force that led to high predominance of MAD20 allelic variant revealed in such malaria declining region demands further research.
Identifiants
pubmed: 34579727
doi: 10.1186/s12936-021-03914-9
pii: 10.1186/s12936-021-03914-9
pmc: PMC8477549
doi:
Substances chimiques
Merozoite Surface Protein 1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
385Informations de copyright
© 2021. The Author(s).
Références
Bioinformatics. 2012 Oct 1;28(19):2537-9
pubmed: 22820204
Mol Cell Probes. 1995 Jun;9(3):161-5
pubmed: 7477008
Malar J. 2020 Jun 8;19(1):203
pubmed: 32513191
PLoS One. 2015 Oct 15;10(10):e0140780
pubmed: 26468643
Malar J. 2008 Nov 21;7:241
pubmed: 19025582
PLoS Negl Trop Dis. 2015 May 07;9(5):e0003739
pubmed: 25951184
Sci Transl Med. 2009 Oct 14;1(2):2ra5
pubmed: 20165550
Mol Cell. 1999 Apr;3(4):457-64
pubmed: 10230398
Malar J. 2009 Jun 20;8:135
pubmed: 19545390
Methods Mol Med. 2002;72:189-203
pubmed: 12125116
Malar J. 2015 Feb 14;14:73
pubmed: 25889847
Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1751-5
pubmed: 2000383
BMC Infect Dis. 2009 May 21;9:69
pubmed: 19460160
Am J Trop Med Hyg. 2017 Mar;96(3):630-641
pubmed: 28070005
Microbiol Spectr. 2019 Jul;7(4):
pubmed: 31400095
Malar J. 2020 Mar 18;19(1):115
pubmed: 32188442
BMC Infect Dis. 2018 Aug 29;18(1):432
pubmed: 30157794
Malar J. 2018 Oct 25;17(1):386
pubmed: 30359280
Parasite Epidemiol Control. 2020 Oct 22;11:e00188
pubmed: 33145445
Malar J. 2020 Mar 16;19(1):112
pubmed: 32178679
Malar J. 2019 Jun 11;18(1):193
pubmed: 31185977
Infect Immun. 2006 Feb;74(2):1313-22
pubmed: 16428781
Parasit Vectors. 2017 Jul 4;10(1):322
pubmed: 28676097
PLoS One. 2017 May 19;12(5):e0177559
pubmed: 28542247
PLoS Negl Trop Dis. 2016 Jan 11;10(1):e0004355
pubmed: 26751811
Malar J. 2019 Feb 21;18(1):48
pubmed: 30791901
Parasitology. 2009 Oct;136(12):1445-56
pubmed: 19627632
Malar J. 2017 Jul 27;16(1):301
pubmed: 28750669
Acta Trop. 2015 Aug;148:97-104
pubmed: 25913735
Parasit Vectors. 2016 Jun 27;9(1):362
pubmed: 27350250
J Infect Dis. 2015 Apr 1;211(7):1121-7
pubmed: 25301957
Trop Med Int Health. 2005 Oct;10(10):1060-4
pubmed: 16185241
Parasitol Res. 2013 Apr;112(4):1691-700
pubmed: 23408340
Malar J. 2011 Apr 06;10:79
pubmed: 21470428
Malar J. 2018 Dec 7;17(1):458
pubmed: 30526609
Parasit Vectors. 2018 May 30;11(1):323
pubmed: 29843783
J Infect Dis. 2020 Feb 18;221(5):775-785
pubmed: 31585009
J Parasitol Res. 2016;2016:3074803
pubmed: 27110390
J Biol Chem. 2016 Apr 1;291(14):7703-15
pubmed: 26823464
N Am J Med Sci. 2013 Feb;5(2):95-101
pubmed: 23641369