Curcumin inhibited hepatitis B viral entry through NTCP binding.
Curcumin
/ pharmacology
DNA, Viral
/ isolation & purification
Hep G2 Cells
Hepatitis B
/ prevention & control
Hepatitis B virus
/ drug effects
Humans
Organic Anion Transporters, Sodium-Dependent
/ metabolism
Reinfection
/ prevention & control
Symporters
/ metabolism
Viral Envelope Proteins
/ metabolism
Virus Internalization
/ drug effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 09 2021
27 09 2021
Historique:
received:
28
04
2021
accepted:
06
09
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
29
12
2021
Statut:
epublish
Résumé
Hepatitis B virus (HBV) has been implicated in hepatitis and hepatocellular carcinoma. Current agents (nucleos(t)ide analogs and interferons) could only attenuate HBV infection. A combination of agents targeting different stages of viral life cycle (e.g., entry, replication, and cccDNA stability) was expected to eradicate the infection. Curcumin (CCM) was investigated for inhibitory action toward HBV attachment and internalization. Immortalized hepatocyte-like cells (imHCs), HepaRG and non-hepatic cells served as host cells for binding study with CCM. CCM decreased viral load, HBeAg, HBcAg (infectivity), intracellular HBV DNA, and cccDNA levels. The CCM-induced suppression of HBV entry was directly correlated with the density of sodium-taurocholate co-transporting polypeptide (NTCP), a known host receptor for HBV entry. The site of action of CCM was confirmed using TCA uptake assay. The affinity between CCM and NTCP was measured using isothermal titration calorimetry (ITC). These results demonstrated that CCM interrupted HBV entry and would therefore suppress HBV re-infection.
Identifiants
pubmed: 34580340
doi: 10.1038/s41598-021-98243-x
pii: 10.1038/s41598-021-98243-x
pmc: PMC8476618
doi:
Substances chimiques
DNA, Viral
0
Organic Anion Transporters, Sodium-Dependent
0
Symporters
0
Viral Envelope Proteins
0
sodium-bile acid cotransporter
145420-23-1
Curcumin
IT942ZTH98
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19125Informations de copyright
© 2021. The Author(s).
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