The Genetic Diversity of Rickettsiella Symbionts in Ixodes ricinus Throughout Europe.

Facultative symbionts Ixodes ricinus ecology Rickettsiella Tick-borne bacteria co-infection Tick-borne pathogens

Journal

Microbial ecology
ISSN: 1432-184X
Titre abrégé: Microb Ecol
Pays: United States
ID NLM: 7500663

Informations de publication

Date de publication:
Aug 2022
Historique:
received: 12 05 2021
accepted: 10 09 2021
pubmed: 29 9 2021
medline: 8 9 2022
entrez: 28 9 2021
Statut: ppublish

Résumé

Rickettsiella species are bacterial symbionts that are present in a great variety of arthropod species, including ixodid ticks. However, little is known about their genetic diversity and distribution in Ixodes ricinus, as well as their relationship with other tick-associated bacteria. In this study, we investigated the occurrence and the genetic diversity of Rickettsiella spp. in I. ricinus throughout Europe and evaluated any preferential and antagonistic associations with Candidatus Midichloria mitochondrii and the pathogens Borrelia burgdorferi sensu lato and Borrelia miyamotoi. Rickettsiella spp. were detected in most I. ricinus populations investigated, encompassing a wide array of climate types and environments. The infection prevalence significantly differed between geographic locations and was significantly higher in adults than in immature life stages. Phylogenetic investigations and protein characterization disclosed four Rickettsiella clades (I-IV). Close phylogenetic relations were observed between Rickettsiella strains of I. ricinus and other arthropod species. Isolation patterns were detected for Clades II and IV, which were restricted to specific geographic areas. Lastly, although coinfections occurred, we did not detect significant associations between Rickettsiella spp. and the other tick-associated bacteria investigated. Our results suggest that Rickettsiella spp. are a genetically and biologically diverse facultative symbiont of I. ricinus and that their distribution among tick populations could be influenced by environmental components.

Identifiants

pubmed: 34580739
doi: 10.1007/s00248-021-01869-7
pii: 10.1007/s00248-021-01869-7
pmc: PMC9436858
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

613-626

Subventions

Organisme : Federal Ministry of Education and Research (BMBF)
ID : 01KI1720
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 799609

Informations de copyright

© 2021. The Author(s).

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Auteurs

Aitor Garcia-Vozmediano (A)

Department of Veterinary Sciences, University of Turin, L.go Braccini, 2, 10095, Grugliasco, TO, Italy. aitor.garciavozmediano@unito.it.

Laura Tomassone (L)

Department of Veterinary Sciences, University of Turin, L.go Braccini, 2, 10095, Grugliasco, TO, Italy.

Manoj Fonville (M)

Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, 3720 BA, Bilthoven, The Netherlands.

Luigi Bertolotti (L)

Department of Veterinary Sciences, University of Turin, L.go Braccini, 2, 10095, Grugliasco, TO, Italy.

Dieter Heylen (D)

Eco-Epidemiology Group, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
Evolutionary Ecology Group, Department of Biology, University of Antwerp, Wilrijk, Belgium.
Interuniversity Institute for Biostatistics and Statistical Bioinformatics, Hasselt University, Diepenbeek, Belgium.

Nannet D Fabri (ND)

Department of Wildlife, Fish, and Environmental Studies, Swedish University of Agricultural Sciences, 901 83, Umeå, Sweden.
Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CL, Utrecht, The Netherlands.

Jolyon M Medlock (JM)

Infections Medical Entomology & Zoonoses Ecology, Public Health England, Porton Down, UK.

Ard M Nijhof (AM)

Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163, Berlin, Germany.

Kayleigh M Hansford (KM)

Infections Medical Entomology & Zoonoses Ecology, Public Health England, Porton Down, UK.

Hein Sprong (H)

Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, 3720 BA, Bilthoven, The Netherlands.

Aleksandra I Krawczyk (AI)

Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, 3720 BA, Bilthoven, The Netherlands. aleksandra.i.krawczyk@gmail.com.
Laboratory of Entomology, Wageningen University and Research Centre, Wageningen, The Netherlands. aleksandra.i.krawczyk@gmail.com.

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