The human sperm basal body is a complex centrosome important for embryo preimplantation development.
centriole
centrosome
compaction
embryo early development
fertilization
human
microtubule organizing centers
pericentriolar material
Journal
Molecular human reproduction
ISSN: 1460-2407
Titre abrégé: Mol Hum Reprod
Pays: England
ID NLM: 9513710
Informations de publication
Date de publication:
02 11 2021
02 11 2021
Historique:
received:
15
04
2021
revised:
09
09
2021
pubmed:
29
9
2021
medline:
16
3
2022
entrez:
28
9
2021
Statut:
ppublish
Résumé
The mechanism of conversion of the human sperm basal body to a centrosome after fertilization, and its role in supporting human early embryogenesis, has not been directly addressed so far. Using proteomics and immunofluorescence studies, we show here that the human zygote inherits a basal body enriched with centrosomal proteins from the sperm, establishing the first functional centrosome of the new organism. Injection of human sperm tails containing the basal body into human oocytes followed by parthenogenetic activation, showed that the centrosome contributes to the robustness of the early cell divisions, increasing the probability of parthenotes reaching the compaction stage. In the absence of the sperm-derived centrosome, pericentriolar material (PCM) components stored in the oocyte can form de novo structures after genome activation, suggesting a tight PCM expression control in zygotes. Our results reveal that the sperm basal body is a complex organelle which converts to a centrosome after fertilization, ensuring the early steps of embryogenesis and successful compaction. However, more experiments are needed to elucidate the exact molecular mechanisms of centrosome inheritance in humans.
Identifiants
pubmed: 34581808
pii: 6377343
doi: 10.1093/molehr/gaab062
pmc: PMC8561016
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.
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