Proteins associated with the Arabidopsis thaliana plastid rhomboid-like protein RBL10.
acyl carrier protein
lipid trafficking
membrane biogenesis
plant biochemistry
rhomboid protease
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
revised:
10
09
2021
received:
04
02
2021
accepted:
21
09
2021
pubmed:
29
9
2021
medline:
8
2
2022
entrez:
28
9
2021
Statut:
ppublish
Résumé
Rhomboid-like proteins are intramembrane proteases with a variety of regulatory roles in cells. Though many rhomboid-like proteins are predicted in plants, their detailed molecular mechanisms or cellular functions are not yet known. Of the 13 predicted rhomboids in Arabidopsis thaliana, one, RBL10, affects lipid metabolism in the chloroplast, because in the respective rbl10 mutant the transfer of phosphatidic acid through the inner envelope membrane is disrupted. Here we show that RBL10 is part of a high-molecular-weight complex of 250 kDa or greater in size. Nine likely components of this complex are identified by two independent methods and include Acyl Carrier Protein 4 (ACP4) and Carboxyltransferase Interactor1 (CTI1), which have known roles in chloroplast lipid metabolism. The acp4 mutant has decreased C16:3 fatty acid content of monogalactosyldiacylglycerol, similar to the rbl10 mutant, prompting us to offer a mechanistic model of how an interaction between ACP4 and RBL10 might affect chloroplast lipid assembly. We also demonstrate the presence of a seventh transmembrane domain in RBL10, refining the currently accepted topology of this protein. Taken together, the identity of possible RBL10 complex components as well as insights into RBL10 topology and distribution in the membrane provide a stepping-stone towards a deeper understanding of RBL10 function in Arabidopsis lipid metabolism.
Identifiants
pubmed: 34582071
doi: 10.1111/tpj.15514
pmc: PMC9219029
mid: NIHMS1814442
doi:
Substances chimiques
Arabidopsis Proteins
0
Galactolipids
0
monogalactosyldiacylglycerol
0
Phosphatidic Acids
0
RBL10 protein, Arabidopsis
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1332-1345Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM110523
Pays : United States
Informations de copyright
© 2021 Society for Experimental Biology and John Wiley & Sons Ltd.
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