A bimolecular fluorescence complementation flow cytometry screen for membrane protein interactions.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 09 2021
28 09 2021
Historique:
received:
06
07
2021
accepted:
14
09
2021
entrez:
29
9
2021
pubmed:
30
9
2021
medline:
29
12
2021
Statut:
epublish
Résumé
Interactions between membrane proteins within a cellular environment are crucial for all living cells. Robust methods to screen and analyse membrane protein complexes are essential to shed light on the molecular mechanism of membrane protein interactions. Most methods for detecting protein:protein interactions (PPIs) have been developed to target the interactions of soluble proteins. Bimolecular fluorescence complementation (BiFC) assays allow the formation of complexes involving PPI partners to be visualized in vivo, irrespective of whether or not these interactions are between soluble or membrane proteins. In this study, we report the development of a screening approach which utilizes BiFC and applies flow cytometry to characterize membrane protein interaction partners in the host Saccharomyces cerevisiae. These data allow constructive complexes to be discriminated with statistical confidence from random interactions and potentially allows an efficient screen for PPIs in vivo within a high-throughput setup.
Identifiants
pubmed: 34584201
doi: 10.1038/s41598-021-98810-2
pii: 10.1038/s41598-021-98810-2
pmc: PMC8478939
doi:
Substances chimiques
Luminescent Proteins
0
Membrane Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19232Informations de copyright
© 2021. The Author(s).
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