DNA hypomethylation leads to cGAS-induced autoinflammation in the epidermis.


Journal

The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664

Informations de publication

Date de publication:
15 11 2021
Historique:
revised: 28 08 2021
received: 13 03 2021
accepted: 31 08 2021
pubmed: 30 9 2021
medline: 4 1 2022
entrez: 29 9 2021
Statut: ppublish

Résumé

DNA methylation is a fundamental epigenetic modification, important across biological processes. The maintenance methyltransferase DNMT1 is essential for lineage differentiation during development, but its functions in tissue homeostasis are incompletely understood. We show that epidermis-specific DNMT1 deletion severely disrupts epidermal structure and homeostasis, initiating a massive innate immune response and infiltration of immune cells. Mechanistically, DNA hypomethylation in keratinocytes triggered transposon derepression, mitotic defects, and formation of micronuclei. DNA release into the cytosol of DNMT1-deficient keratinocytes activated signaling through cGAS and STING, thus triggering inflammation. Our findings show that disruption of a key epigenetic mark directly impacts immune and tissue homeostasis, and potentially impacts our understanding of autoinflammatory diseases and cancer immunotherapy.

Identifiants

pubmed: 34586646
doi: 10.15252/embj.2021108234
pmc: PMC8591534
doi:

Substances chimiques

Adaptor Proteins, Signal Transducing 0
IPS-1 protein, mouse 0
Membrane Proteins 0
Sting1 protein, mouse 0
DNA (Cytosine-5-)-Methyltransferase 1 EC 2.1.1.37
Dnmt1 protein, mouse EC 2.1.1.37
Nucleotidyltransferases EC 2.7.7.-
cGAS protein, mouse EC 2.7.7.-
Ifih1 protein, mouse EC 3.6.1.-
Interferon-Induced Helicase, IFIH1 EC 3.6.4.13

Banques de données

GEO
['GSE166356']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e108234

Subventions

Organisme : Austrian Science Fund FWF
ID : P 28034
Pays : Austria

Informations de copyright

© 2021 The Authors. Published under the terms of the CC BY 4.0 license.

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Auteurs

Mirjam A Beck (MA)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Heinz Fischer (H)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Lisa M Grabner (LM)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Tamara Groffics (T)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Mircea Winter (M)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Simone Tangermann (S)

Unit of Laboratory Animal Pathology, University of Veterinary Medicine, Vienna, Austria.

Tina Meischel (T)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Barbara Zaussinger-Haas (B)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Patrick Wagner (P)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Carina Fischer (C)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Christina Folie (C)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.
Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Salzburg, Austria.

Julia Arand (J)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Christian Schöfer (C)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Bernard Ramsahoye (B)

MRC Institute of Genetics & Molecular Medicine, Centre for Genomic & Experimental Medicine, University of Edinburgh, Edinburgh, UK.

Sabine Lagger (S)

Unit of Laboratory Animal Pathology, University of Veterinary Medicine, Vienna, Austria.

Georg Machat (G)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Gregor Eisenwort (G)

Institute of Immunology, Medical University of Vienna, Vienna, Austria.

Stephanie Schneider (S)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Alexandra Podhornik (A)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Michael Kothmayer (M)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

Ursula Reichart (U)

VetCORE - Facility for Research, University of Veterinary Medicine Vienna, Vienna, Austria.

Martin Glösmann (M)

VetCORE - Facility for Research, University of Veterinary Medicine Vienna, Vienna, Austria.

Ido Tamir (I)

Campus Science Support Facilities GmbH, Vienna, Austria.

Michael Mildner (M)

Research Division of Biology and Pathobiology of the Skin, Medical University of Vienna, Vienna, Austria.

Raheleh Sheibani-Tezerji (R)

Ludwig Boltzmann Institute Applied Diagnostics, Vienna, Austria.

Lukas Kenner (L)

Unit of Laboratory Animal Pathology, University of Veterinary Medicine, Vienna, Austria.
Christian Doppler Laboratory for Applied Metabolomics, Vienna, Austria.
Department of Pathology, Medical University of Vienna, Vienna, Austria.
CBmed GmbH - Center for Biomarker Research in Medicine, Graz, Austria.

Peter Petzelbauer (P)

Skin and Endothelium Research Division, Department of Dermatology, Medical University of Vienna, Vienna, Austria.

Gerda Egger (G)

Ludwig Boltzmann Institute Applied Diagnostics, Vienna, Austria.
Department of Pathology, Medical University of Vienna, Vienna, Austria.

Maria Sibilia (M)

Department of Medicine I, Comprehensive Cancer Center, Institute of Cancer Research, Medical University of Vienna), Vienna, Austria.

Andrea Ablasser (A)

Global Health Institute, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.

Christian Seiser (C)

Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

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Classifications MeSH