Detection of Endogenous DNA Double-strand Breaks in Oral Squamous Epithelial Lesions by P53-binding Protein 1.
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor
/ metabolism
Carcinoma, Squamous Cell
/ metabolism
Cell Nucleus
/ metabolism
DNA Breaks, Double-Stranded
Disease Progression
Female
Genomic Instability
Humans
Ki-67 Antigen
/ metabolism
Male
Middle Aged
Mouth Diseases
/ metabolism
Mouth Neoplasms
/ metabolism
Squamous Intraepithelial Lesions
/ metabolism
Tumor Suppressor p53-Binding Protein 1
/ metabolism
53BP1
genome instability
immunofluorescence
oral cancer
tumor biomarkers
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
27
06
2021
revised:
24
07
2021
accepted:
24
08
2021
entrez:
1
10
2021
pubmed:
2
10
2021
medline:
9
10
2021
Statut:
ppublish
Résumé
P53-binding protein 1 (53BP1) is one of the DNA damage response (DDR) molecules. This study aimed to assess 53BP1 expression by immunofluorescence (IF) as a biomarker to differentiate between oral squamous epithelial lesions (OSELs). We analyzed 129 archival oral biopsy samples, including 18 benign squamous lesions (BSLs), 37 low-grade dysplasias (LGDs), 22 high-grade dysplasias (HGDs), and 52 oral squamous cell carcinomas (OSCCs). 53BP1 and Ki-67 expressions were examined by double IF to assess the type of 53BP1 expression. We found that OSCC exhibited several 53BP1 nuclear foci, particularly high-DNA damage response (HDDR) and large focus (LF)-type, suggesting the presence of endogenous DNA double-strand breaks in the cancer genome, which could disrupt DDR and induce genomic injury. We also found a difference in 53BP1 expression between LGD and HGD, but not between BSL and LGD. Among the Ki-67-positive cells, HDDR- and LF-type expressions were higher in OSELs of higher grades. 53BP1 expression can be a valuable biomarker for OSELs to help estimate the grade of oral epithelial dysplasia.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
P53-binding protein 1 (53BP1) is one of the DNA damage response (DDR) molecules. This study aimed to assess 53BP1 expression by immunofluorescence (IF) as a biomarker to differentiate between oral squamous epithelial lesions (OSELs).
MATERIALS AND METHODS
METHODS
We analyzed 129 archival oral biopsy samples, including 18 benign squamous lesions (BSLs), 37 low-grade dysplasias (LGDs), 22 high-grade dysplasias (HGDs), and 52 oral squamous cell carcinomas (OSCCs). 53BP1 and Ki-67 expressions were examined by double IF to assess the type of 53BP1 expression.
RESULTS
RESULTS
We found that OSCC exhibited several 53BP1 nuclear foci, particularly high-DNA damage response (HDDR) and large focus (LF)-type, suggesting the presence of endogenous DNA double-strand breaks in the cancer genome, which could disrupt DDR and induce genomic injury. We also found a difference in 53BP1 expression between LGD and HGD, but not between BSL and LGD. Among the Ki-67-positive cells, HDDR- and LF-type expressions were higher in OSELs of higher grades.
CONCLUSION
CONCLUSIONS
53BP1 expression can be a valuable biomarker for OSELs to help estimate the grade of oral epithelial dysplasia.
Identifiants
pubmed: 34593426
pii: 41/10/4771
doi: 10.21873/anticanres.15292
doi:
Substances chimiques
Biomarkers, Tumor
0
Ki-67 Antigen
0
TP53BP1 protein, human
0
Tumor Suppressor p53-Binding Protein 1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4771-4779Informations de copyright
Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.