In vitro Inhibition of respiratory pathogens by lactobacillus and alpha haemolytic streptococci from Aboriginal and Torres Strait Islander children.
Indigenous
Lactobacillus
alpha haemolytic streptococcus
bacterial interference
in vitro
otitis media
probiotic
respiratory pathogens
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
08
07
2021
received:
30
12
2020
accepted:
29
07
2021
pubmed:
5
10
2021
medline:
24
2
2022
entrez:
4
10
2021
Statut:
ppublish
Résumé
To explore the in vitro ability of alpha haemolytic streptococcus (AHS) and lactobacilli (LBs), from Indigenous Australian children, to inhibit the growth of respiratory pathogens (Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis), also from Indigenous Australian children. The bacterial interference of 91 isolates, from Indigenous Australian children both with and without otitis media (OM) or rhinorrhoea, was investigated using agar overlay and cell-free supernatant. Promising isolates underwent whole genome sequencing to investigate upper respiratory tract tropism, antibiotic resistance and virulence. Antibiotic susceptibility was examined for ampicillin, amoxicillin +clavulanic acid and azithromycin. Differences in the strength of bacterial inferences in relation to OM was examined using a case series of three healthy and three children with OM. LBs readily inhibited the growth of pathogens. AHS were less effective, although several isolates inhibited S. pneumoniae. One L. rhamnosus had genes coding for pili to adhere to epithelial cells. We detected antibiotic resistance genes coding for antibiotic efflux pump and ribosomal protection protein. LBs were susceptible to antimicrobials in vitro. Screening for virulence detected genes encoding for two putative capsule proteins. Healthy children had AHS and LB that were more potent inhibitors of respiratory pathogens in vitro than children with OM. L. rhamnosus from remote Indigenous Australian children are potent inhibitors of respiratory pathogens in vitro. Respiratory/ear disease are endemic in Indigenous Australians. There is an urgent call for more effective treatment/prevention; beneficial microbes have not been explored. L. rhamnosus investigated in this study are potent inhibitors of respiratory pathogens in vitro and require further investigation.
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2368-2378Subventions
Organisme : Queensland Health Junior Doctor Fellowship
Organisme : Avant Doctors in Training Research Scholarship
Organisme : The University of Queensland Faculty of Medicine Strategic Funding
Organisme : National Health and Medical Research Council
ID : APP1133366
Informations de copyright
© 2021 The Society for Applied Microbiology.
Références
Azcarate-Peril, M., McAuliffe, O., Altermann, E., Lick, S., Russell, M. & Klaenhammer, T. (2005) Microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in Lactobacillus acidophilus. Applied and Environment Microbiology, 71, 5794-5804.
Bill, N.J. & Washington, J.A. (1975) Bacterial Interference by Streptococcus Salivaris. American Journal of Clinical Pathology, 64, 116-120.
Bogaert, D., Keijser, B., Huse, S., Rossen, J., Veenhoven, R., van Gils, E. et al. (2011) Variability and diversity of nasopharyngeal microbiota in children: a metagenomic analysis. PLoS One, 6, e17035.
Bosch, A., Biesbroek, G., Trzcinski, K., Sanders, E. & Bogaert, D. (2013) Viral and bacterial interactions in the upper respiratory tract. PLoS Path, 9, e1003057.
Brook, I. (2005) The Role of Bacterial Interference in Otitis, Sinusitis and Tonsillitis. Otolaryngology-head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery, 133, 139-146.
Brook, I. & Gober, A. (2000) In vitro bacterial interference in the nasopharynx of otitis media-prone and non-otitis-media-prone chlidren. Archives of Otolaryngology - Head and Neck Surgery, 126, 1011-1013.
Campedelli, I., Mathur, H., Salvetti, E., Clarke, S., Rea, M., Torriani, S. et al. (2019) Genus-wide assessment of antibiotic resistance in Lactobacillus spp. Applied and Environment Microbiology, 85, e01738-e11718.
Clinical and Laboratory Standards Institute. (2015) Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria. Wayne, USA: Clinical and Laboratory Standards Istitute.
Coleman, A., Bialasiewicz, S., Marsh, R., Grahn Håkansson, E., Cottrell, K., Wood, A. et al. (2021) Upper respiratory microbiota in relation to ear and nose health among Australian Aboriginal and Torres Strait Islander children. Journal of the Pediatric Infectious Diseases Society, 10(4), 468-476.
Coleman, A. & Cervin, A. (2019) Probiotics in the treatment of otitis media. The past, the present and the future. International Journal of Pediatric Otorhinolaryngology, 116, 135-140.
Coticchia, J.M., Chen, M., Sachdeva, L. & Mutchnick, S. (2013) New paradigms in the pathogenesis of otitis media in children. Frontiers in Pediatrics, 1, 52.
Cotter, P., Emerson, N., Gahan, C. & Hill, C. (1999) Identification and disruption of lisRK, a genetic locus encoding a two-component signal transduction system involved in stress tolerance and tirulence in Listeria monocytogenes. Journal of Bacteriology, 18, 6840-6843.
De Boeck, I., van den Broek, M.F.L., Allonsius, C.N., Spacova, I., Wittouck, S., Martens, K. et al. (2020) Lactobacilli have a niche in the human nose. Cell Reports, 31, 107674.
Hoa, Q., Rong Dong, B. & Wu, T. (2015) Probiotics for preventing acute upper respiratory tract infections. Cochrane Database Systematic Review.
Kong, K. & Coates, H.L.C. (2009) Natural history, definitions, risk factors and burden of otitis media. Medical Journal of Australia, 191, S39-S43.
Kumpu, M., Swanljung, E., Tynkkynen, S., Hatakka, K., Kekkonen, R., Järvenpää, S. et al. (2013) Recovery of probiotic Lactobacillus rhamnosus GG in tonsil tissue after oral administration: randomised, placebo-controlled, double-blind clinical trial. British Journal of Nutrition, 190, 2240-2246.
Leach, A.J., Boswell, J., Asche, V., Nienhuys, T. & Matthews, J. (1994) Bacterial colonization of the nasopharynx predicts very early onset and persistence of otitis media in Australian aboriginal infants. The Pediatric Infectious Disease Journal, 13, 983-989.
McFarland, L., Evans, C. & Goldstein, E. (2018) Strain-specificity and disease-specificity of probiotic efficacy: a systematic review and meta-analysis. Frontiers in Medicine, 5, 124.
Monasta, L., Ronfan, L., Marchetti, F., Monico, M., Vecchi Brumatti, L., Bavcar, A. et al. (2012) Burden of disease caused by otitis media: systematic review and global estimates. PLoS One, 7, e36226.
Morris, P.S., Leach, A.J., Silberberg, P., Mellon, G., Wilson, C., Hamilton, E. et al. (2004) Otitis media in young Aboriginal children from remote communities in Northern and Central Australia: a cross-sectional survey. BMC Pediatrics, 5, 27.
Pearce, C., Bowden, G., Evans, M., Fitzsimmons, S., Johnson, J., Sheridan, M. et al. (1995) Identification of pioneer viridans streptococci in the oral cavity of human neonates. Journal of Medical Microbiology, 42, 67-72.
Roos, K., Grahn Håkansson, E. & Holm, S. (2001) Effect of recolonisation with “interfering” alpha streptococci on recurrences of acute and secretory otitis media in children: randomised placebo controlled trial. BMJ, 322, 210-212.
Santagati, M., Scillato, M., Patanè, F., Aiello, C. & Stefani, S. (2012) Bacteriocin-producing oral streptococci and inhibition of respiratory pathogens. FEMS Immunology and Medical Microbiology, 65, 23-31.
Savijoki, K., Nyman, T., Kainulainen, V., Miettinen, I., Siljamäki, P., Fallarero, A. et al. (2019) Growth mode and carbon source impact the surfaceome dynamics of Lactobacillus rhamnosus GG. Frontiers in Microbiology, 10, 1272.
Scott, A., Clark, J., Julien, B., Islam, F., Roos, K., Grimwood, K. et al. (2019) Probiotics for preventing acute otitis media in children. Cochrane Database of Systematic Reviews, 2019, CD012941.
Sharma, P., Tomar, S., Goswami, P., Sangwan, V. & Singh, R. (2014) Antibiotic resistance among commercially available probiotics. Food Research International, 57, 176-195.
Skovbjerg, S., Roos, K., Holm, S., Grahn Hakansson, E., Nowrouzian, F., Ivarsson, M. et al. (2008) Spray bacteriotherapy decreases middle ear fluid in children with secretory otitis media. Archives of Disease in Childhood, 94, 92-98.
Stearns, J., Davidson, C., McKeon, S., Whelan, F., Fontes, M., Schryvers, A. et al. (2015) Culture and molecular-based profiles show shifts in bacterial communities of the upper respiratory tract that occur with age. ISME Journal, 9, 1246-1259.
Swanljung, E., Tapiovaara, L., Lehtoranta, L., Mäkivuokko, H., Roivainen, M., Korpela, R. et al. (2015) Lactobacillus rhamnosus GG in adenoid tissue: double-blind, placebo-controlled, randomized clinical trial. Acta Otolaryngo, 135, 824-830.
Tano, K., Grahn-Håkansson, E., Holm, S. & Hellström, S. (2000) Inhibition of OM pathogens by alpha-hemolytic streptococci from healthy children, children with SOM and children with rAOM. International Journal of Pediatric Otorhinolaryngology, 56, 185-190.
Tano, K., Grahn-Håkansson, E., Holm, S. & Hellström, S. (2002) Bacterial interference between otitis media pathogens and alpha-haemolytic streptococci analysed in an in vitro model. Acta Otolaryngo, 122, 78-85.
Tano, K., Grahn-Håkansson, E., Wallbrandt, P., Rönnqvist, D., Holm, S. & Hellström, S. (2003) Is hydrogen peroxide responsible for the inhibitory activity of α-haemolytic Streptococci sampled from the nasopharynx? Acta Otolaryngo, 123, 724-729.
Tano, K., Olofsson, C., Grahn Håkansson, E. & Holm, S. (1999) In vitro inhibition of S. pneumoniae, nontypable H. influenzae and M. catarrhalis by alpha-haemolytic streptococci from healthy children. International Journal of Pediatric Otorhinolaryngology, 47, 49-56.
Tapiovaara, L., Lehtoranta, L., Swanljung, E., Mäkivuokko, H., Laakso, S., Roivainen, M. et al. (2014) Lactobacillus GG in the middle ear after randomized, double-blind, placebo-controlled oral administration. International Journal of Pediatric Otorhinolaryngology, 78, 1637-1641.
Tapiovaara, L., Pitkaranta, A. & Korpela, R. (2016) Probiotics and the upper respiratory tract - a review. Pediatric Infectious Diseases: Open Access, 01, 19.
van den Broek, M., De Boeck, I., Claes, I., Nizet, V. & Lebeer, S. (2018) Multifactorial inhibition of lactobacillus against the respiratory tract pathogen Moraxella catarrhalis. Benef Microbes, 9, 429-439.
van den Broek, M., De Boeck, I., Kiekens, K., Boudewyns, A., Vanderveken, O. & Lebeer, S. (2019) Translating recent microbiome insights in otitis media into probiotic strategies. Clinical Microbiology Reviews, 32, 00010-00018.
Wuyts, S., Allonsius, C., Wittouck, S., Thys, S., Lievens, B., Weckx, S. et al. (2019) Comparative genome analysis of Lactobacillus mudanjiangensis, an understudied member of the Lactobacillus plantarum group. Microbial Genomics, 5, PMC6807380.
Wuyts, S., Wittouck, S., De Boeck, I., Allonsius, C., Pasolli, E., Segata, N. et al. (2017) Large-scale phylogenomics of the lactobacillus casei group highlights taxonomic inconsistencies and reveals novel clade-associated features, mSystems, 2, e00061-e17.
Zheng, J., Wittouck, S., Salvetti, E., Franz, C., Harris, H., Mattarelli, P. et al. (2020) A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. International Journal of Systematic and Evolutionary Microbiology, 70, 2782-2858.