KPC-39-Mediated Resistance to Ceftazidime-Avibactam in a Klebsiella pneumoniae ST307 Clinical Isolate.
KPC
avibactam resistance
carbapenemase
Journal
Antimicrobial agents and chemotherapy
ISSN: 1098-6596
Titre abrégé: Antimicrob Agents Chemother
Pays: United States
ID NLM: 0315061
Informations de publication
Date de publication:
17 11 2021
17 11 2021
Historique:
pubmed:
5
10
2021
medline:
15
12
2021
entrez:
4
10
2021
Statut:
ppublish
Résumé
Resistance to the ceftazidime (CAZ)-avibactam (AVI) combination is increasingly being reported. Here, we report a CAZ-AVI-resistant Klebsiella pneumoniae strain belonging to the high-risk sequence type 307 (ST307) clone and producing Klebsiella pneumoniae carbapenemase 39 (KPC-39), a single-amino-acid variant of KPC-3 (A172T). Cloning experiments, steady-state kinetic parameters, and molecular dynamics simulations revealed a loss of carbapenemase activity and increased affinity for CAZ. KPC-39 was identified in a patient without prior exposure to CAZ-AVI, suggesting silent dissemination in European health care settings.
Identifiants
pubmed: 34606331
doi: 10.1128/AAC.01160-21
pmc: PMC8597731
doi:
Substances chimiques
Anti-Bacterial Agents
0
Azabicyclo Compounds
0
Bacterial Proteins
0
Drug Combinations
0
avibactam, ceftazidime drug combination
0
Ceftazidime
9M416Z9QNR
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0116021Subventions
Organisme : Université Paris-Saclay (University of Paris-Saclay)
Organisme : Assistance Publique - Hôpitaux de Paris (AP-HP)
Organisme : Agence Nationale de la Recherche (ANR)
ID : ANR-10-LABX-33
Organisme : Agence Nationale de la Recherche (ANR)
ID : ANR-19-AMRB-0004
Organisme : Institut National de la Santé et de la Recherche Médicale (Inserm)
ID : 1184
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