Counting actin in contractile rings reveals novel contributions of cofilin and type II myosins to fission yeast cytokinesis.
Journal
Molecular biology of the cell
ISSN: 1939-4586
Titre abrégé: Mol Biol Cell
Pays: United States
ID NLM: 9201390
Informations de publication
Date de publication:
15 05 2022
15 05 2022
Historique:
pubmed:
7
10
2021
medline:
18
5
2022
entrez:
6
10
2021
Statut:
ppublish
Résumé
Cytokinesis by animals, fungi, and amoebas depends on actomyosin contractile rings, which are stabilized by continuous turnover of actin filaments. Remarkably little is known about the amount of polymerized actin in contractile rings, so we used low concentrations of GFP-Lifeact to count total polymerized actin molecules in the contractile rings of live fission yeast cells. Contractile rings of wild-type cells accumulated polymerized actin molecules at 4900/min to a peak number of ∼198,000 followed by a loss of actin at 5400/min throughout ring constriction. In
Identifiants
pubmed: 34613787
doi: 10.1091/mbc.E21-08-0376
pmc: PMC9265160
doi:
Substances chimiques
Actin Depolymerizing Factors
0
Actins
0
MYO2 protein, S pombe
0
Myp2 protein, S pombe
0
Schizosaccharomyces pombe Proteins
0
Myosin Type II
EC 3.6.1.-
Myosin Heavy Chains
EC 3.6.4.1
Myosins
EC 3.6.4.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
ar51Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM026132
Pays : United States
Organisme : NIGMS NIH HHS
ID : R15 GM134496
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM139337
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS118884
Pays : United States
Références
Genes Cells. 2006 Aug;11(8):893-905
pubmed: 16866873
Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):E5876-E5885
pubmed: 27647921
J Cell Sci. 1989 Dec;94 ( Pt 4):647-56
pubmed: 2630561
Curr Biol. 2017 Feb 6;27(3):R99-R100
pubmed: 28171765
Mol Biol Cell. 2019 Apr 1;30(8):933-941
pubmed: 30759055
J Cell Biol. 1999 Jul 26;146(2):453-64
pubmed: 10427097
Mol Biol Cell. 2019 Jul 15;30(15):1791-1804
pubmed: 31116668
J Cell Biol. 2007 Sep 24;178(7):1251-64
pubmed: 17875745
Genes Dev. 2013 Oct 1;27(19):2164-77
pubmed: 24115772
PLoS Biol. 2016 Apr 15;14(4):e1002437
pubmed: 27082518
Science. 2008 Jan 4;319(5859):97-100
pubmed: 18079366
Dev Cell. 2014 Jun 9;29(5):547-561
pubmed: 24914559
Mol Biol Cell. 2014 Jul 1;25(13):1946-57
pubmed: 24790095
J Struct Biol. 2012 Jan;177(1):32-9
pubmed: 22056467
Curr Biol. 2005 Apr 26;15(8):724-31
pubmed: 15854904
Nat Methods. 2014 Jul;11(7):731-3
pubmed: 24859753
J Cell Biol. 2003 Jun 9;161(5):875-87
pubmed: 12796476
Science. 2005 Oct 14;310(5746):310-4
pubmed: 16224022
Curr Biol. 2000 Apr 6;10(7):397-400
pubmed: 10753748
Elife. 2018 Jul 02;7:
pubmed: 29963981
Curr Biol. 2005 Apr 26;15(8):732-6
pubmed: 15854905
Science. 2003 Mar 14;299(5613):1743-7
pubmed: 12637748
J Cell Biol. 2013 Oct 14;203(1):101-14
pubmed: 24127216
Nat Cell Biol. 2016 Jun;18(6):676-83
pubmed: 27159499
Nat Commun. 2017 Sep 26;8(1):703
pubmed: 28951543
J Cell Biol. 1972 May;53(2):419-34
pubmed: 5063470
J Cell Biol. 1990 Apr;110(4):1089-95
pubmed: 2324193
IEEE Trans Image Process. 1998;7(1):27-41
pubmed: 18267377
Curr Biol. 2013 Jul 8;23(13):1154-62
pubmed: 23727096
J Theor Biol. 1983 Mar 21;101(2):289-316
pubmed: 6683772
Annu Rev Biochem. 2019 Jun 20;88:661-689
pubmed: 30649923
Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1455-E1464
pubmed: 29348205
Dev Cell. 2003 Nov;5(5):723-34
pubmed: 14602073
J Cell Biol. 2011 Oct 31;195(3):485-98
pubmed: 22024167
J Cell Sci. 2015 Aug 1;128(15):2903-18
pubmed: 26092938
Curr Biol. 2015 Aug 3;25(15):1955-65
pubmed: 26144970
Nat Methods. 2008 Jul;5(7):605-7
pubmed: 18536722
J Cell Sci. 1996 Jan;109 ( Pt 1):131-42
pubmed: 8834798
J Cell Biol. 2012 Nov 26;199(5):831-47
pubmed: 23185032
Science. 2004 Apr 30;304(5671):743-6
pubmed: 15118165
iScience. 2019 Apr 26;14:69-87
pubmed: 30928696
J Cell Biol. 1990 Nov;111(5 Pt 1):1905-11
pubmed: 2229180
Mol Biol Cell. 2012 Dec;23(24):4833-48
pubmed: 23097492