Culture with apically applied healthy or disease sputum alters the airway surface liquid proteome and ion transport across human bronchial epithelial cells.
CFTR
epithelial cell
epithelium
ion transport
proteomics
sputum
Journal
American journal of physiology. Cell physiology
ISSN: 1522-1563
Titre abrégé: Am J Physiol Cell Physiol
Pays: United States
ID NLM: 100901225
Informations de publication
Date de publication:
01 12 2021
01 12 2021
Historique:
pubmed:
7
10
2021
medline:
15
12
2021
entrez:
6
10
2021
Statut:
ppublish
Résumé
Airway secretions contain many signaling molecules and peptides/proteins that are not found in airway surface liquid (ASL) generated by normal human bronchial epithelial cells (NHBEs) in vitro. These play a key role in innate defense and mediate communication between the epithelium, the immune cells, and the external environment. We investigated how culture of NHBE with apically applied secretions from healthy or diseased (cystic fibrosis, CF) lungs affected epithelial function with a view to providing better in vitro models of the in vivo environment. NHBEs from 6 to 8 different donors were cultured at air-liquid interface (ALI), with apically applied sputum from normal healthy donors (normal lung sputum; NLS) or CF donors (CFS) for 2-4 h, 48 h, or with sputum reapplied over 48 h. Proteomics analysis was carried out on the sputa and on the NHBE ASL before and after culture with sputa. Transepithelial electrical resistance (TEER), short circuit current (I
Identifiants
pubmed: 34613844
doi: 10.1152/ajpcell.00234.2021
pmc: PMC8714986
doi:
Substances chimiques
Membrane Transport Proteins
0
Proteome
0
Banques de données
figshare
['10.6084/m9.figshare.14791827']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
C954-C963Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK065988
Pays : United States
Références
J Clin Invest. 1998 Sep 15;102(6):1125-31
pubmed: 9739046
Methods Mol Biol. 2013;945:109-21
pubmed: 23097104
Proc Am Thorac Soc. 2010 Nov;7(6):387-94
pubmed: 21030518
Am J Respir Cell Mol Biol. 2014 Mar;50(3):502-12
pubmed: 24074402
FASEB J. 2005 Jun;19(8):969-70
pubmed: 15809358
Eur Respir J. 2018 Oct 4;52(4):
pubmed: 30190268
Am J Physiol Lung Cell Mol Physiol. 2007 Jul;293(1):L96-L104
pubmed: 17384080
Am J Respir Crit Care Med. 2013 Jan 15;187(2):170-9
pubmed: 23220915
Mediators Inflamm. 2015;2015:626530
pubmed: 26185365
Nat Methods. 2009 May;6(5):359-62
pubmed: 19377485
Am J Physiol Lung Cell Mol Physiol. 2011 Oct;301(4):L575-86
pubmed: 21743026
Br J Pharmacol. 2004 Feb;141(4):698-708
pubmed: 14744818
Pflugers Arch. 2012 Oct;464(4):353-65
pubmed: 22864553
PLoS One. 2015 Sep 28;10(9):e0138988
pubmed: 26414376
Eur Respir J. 2017 Jul 5;50(1):
pubmed: 28679606
J Biol Chem. 2009 May 29;284(22):14904-13
pubmed: 19321437
Annu Rev Pathol. 2012;7:267-82
pubmed: 22017581
Thorax. 1992 Jan;47(1):25-9
pubmed: 1539140
Eur Respir J. 2008 Sep;32(3):783-95
pubmed: 18757703
Am J Respir Crit Care Med. 2010 Jun 15;181(12):1355-66
pubmed: 20194819
J Biol Chem. 2005 Mar 18;280(11):10202-9
pubmed: 15647273
Proc Am Thorac Soc. 2004;1(1):42-6
pubmed: 16113411
Am J Respir Crit Care Med. 2001 Nov 15;164(10 Pt 1):1964-70
pubmed: 11734453
Am J Physiol Cell Physiol. 2016 Aug 1;311(2):C225-36
pubmed: 27306366
J Biol Chem. 2001 Dec 28;276(52):49169-82
pubmed: 11602603
Eur Respir J. 2018 Dec 20;52(6):
pubmed: 30287473
Pflugers Arch. 2010 Jun;460(1):1-17
pubmed: 20401730
J Immunol. 2012 Sep 1;189(5):2460-7
pubmed: 22844115
Am J Physiol Lung Cell Mol Physiol. 2009 May;296(5):L751-62
pubmed: 19270179
Respir Res. 2010 Oct 08;11:141
pubmed: 20932306
Mol Cell Proteomics. 2005 Aug;4(8):1052-60
pubmed: 15901828
Am J Respir Crit Care Med. 2018 Feb 15;197(4):481-491
pubmed: 29099608
J Biol Chem. 2012 Sep 21;287(39):32556-65
pubmed: 22859302
J Clin Invest. 2002 Feb;109(3):363-71
pubmed: 11827996
Genes Dev. 1999 May 15;13(10):1211-33
pubmed: 10346810
Br J Pharmacol. 2007 Aug;151(8):1204-15
pubmed: 17603555
Am Rev Respir Dis. 1992 Jun;145(6):1265-9
pubmed: 1595989
J Med Chem. 2020 Nov 25;63(22):13258-13265
pubmed: 32692176
Am J Physiol Lung Cell Mol Physiol. 2015 Jul 15;309(2):L109-18
pubmed: 26001773
J Gen Physiol. 2009 Apr;133(4):421-38
pubmed: 19289574
Br J Pharmacol. 2005 Oct;146(3):397-407
pubmed: 16025139
Trends Cell Biol. 2004 Jan;14(1):20-8
pubmed: 14729177
Am J Physiol Lung Cell Mol Physiol. 2005 Jan;288(1):L77-83
pubmed: 15361357
Am J Respir Crit Care Med. 1994 Jul;150(1):207-13
pubmed: 7912987