Periapical lesions in two inbred strains of rats differing in immunological reactivity.
Albino Oxford rats
Dark Agouti rats
immune response
periapical lesion
strain differences
Journal
International endodontic journal
ISSN: 1365-2591
Titre abrégé: Int Endod J
Pays: England
ID NLM: 8004996
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
revised:
25
09
2021
received:
16
05
2021
accepted:
27
09
2021
pubmed:
7
10
2021
medline:
15
12
2021
entrez:
6
10
2021
Statut:
ppublish
Résumé
To investigate the influence of strain differences in immune responses on the pathogenesis of experimental periapical lesions in Dark Agouti (DA) and Albino Oxford (AO) inbred strains of rats. Periapical lesions were induced in male DA and AO rats by pulp exposure of the first mandibular right molars to the oral environment. Animals were killed 21 days after pulp exposure. The mandibular jaws were retrieved and prepared for radiographic, pathohistological, immunohistochemical analysis, real-time PCR and flow cytometry. Blood samples and the supernatant of periapical lesions were collected for measurement of cytokines and oxidative stress marker levels. Statistical analysis was performed using the Kruskal-Wallis H and Mann-Whitney U non-parametric tests or parametric One-Way anova and Independent Samples T-test to determine the differences between groups depending on the normality of the data. A significant difference was considered when p values were <.05. DA rats developed significantly larger (p < .05) periapical lesions compared to AO rats as confirmed by radiographic and pathohistological analysis. The immunohistochemical staining intensity for CD3 was significantly greater in periapical lesions of DA rats compared to AO rats (p < .05). In DA rats, periapical lesions had a significantly higher (p < .05) percentage of CD3+ cells compared to AO rats. Also, the percentage of INF-γ, IL-17 and IL-10 CD3+CD4+ cells was significantly higher in DA rats (p < .05). DA rats had a significantly higher Th17/Th10 ratio. RT-PCR expression of IL-1β, INF-γ and IL-17 genes was significantly higher in periapical lesions of DA compared to AO rats (p < .05). The receptor activator of nuclear factor kappa-Β ligand/osteoprotegerin ratio was higher in DA compared to AO rats with periapical lesions (p < .05). Systemic levels of TNF-α and IL-6 were significantly higher in DA compared to AO rats (p < .05). Levels of lipid peroxidation measured as thiobarbituric acid reactive substances and reduced glutathione were significantly higher (p < .05) in the supernatant in the periapical lesions of DA rats. After pulp exposure, DA rats developed much larger periapical lesions compared to AO rats. Genetically determined differences in immunopathology have been demonstrated to be a significant element defining the severity of periapical lesions.
Substances chimiques
Bone Density Conservation Agents
0
Tumor Necrosis Factor-alpha
0
Types de publication
Journal Article
Langues
eng
Pagination
64-78Subventions
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : ON 175071
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : ON 175103
Organisme : Faculty of Medical Sciences, University of Kragujevac, Serbia
ID : JP 06/20
Organisme : Faculty of Medical Sciences, University of Kragujevac, Serbia
ID : JP 24/20
Organisme : Faculty of Medical Sciences, University of Kragujevac, Serbia
ID : JP 25/19
Informations de copyright
© 2021 International Endodontic Journal. Published by John Wiley & Sons Ltd.
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