Distinct roles of haspin in stem cell division and male gametogenesis.
Animals
Aurora Kinase B
/ metabolism
Cell Differentiation
Cell Division
/ genetics
Cell Self Renewal
/ genetics
Centromere
/ genetics
Gametogenesis
/ genetics
Gene Dosage
Gene Expression Profiling
Gene Knockdown Techniques
Histones
/ metabolism
Intracellular Signaling Peptides and Proteins
/ genetics
Male
Mice
Miosis
/ genetics
Mitosis
Models, Biological
Protein Binding
Protein Serine-Threonine Kinases
/ genetics
Stem Cells
/ cytology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 10 2021
06 10 2021
Historique:
received:
07
07
2021
accepted:
17
09
2021
entrez:
7
10
2021
pubmed:
8
10
2021
medline:
29
12
2021
Statut:
epublish
Résumé
The kinase haspin phosphorylates histone H3 at threonine-3 (H3T3ph) during mitosis. H3T3ph provides a docking site for the Chromosomal Passenger Complex at the centromere, enabling correction of erratic microtubule-chromosome contacts. Although this mechanism is operational in all dividing cells, haspin-null mice do not exhibit developmental anomalies, apart from aberrant testis architecture. Investigating this problem, we show here that mouse embryonic stem cells that lack or overexpress haspin, albeit prone to chromosome misalignment during metaphase, can still divide, expand and differentiate. RNA sequencing reveals that haspin dosage affects severely the expression levels of several genes that are involved in male gametogenesis. Consistent with a role in testis-specific expression, H3T3ph is detected not only in mitotic spermatogonia and meiotic spermatocytes, but also in non-dividing cells, such as haploid spermatids. Similarly to somatic cells, the mark is erased in the end of meiotic divisions, but re-installed during spermatid maturation, subsequent to methylation of histone H3 at lysine-4 (H3K4me
Identifiants
pubmed: 34615946
doi: 10.1038/s41598-021-99307-8
pii: 10.1038/s41598-021-99307-8
pmc: PMC8494884
doi:
Substances chimiques
Haspin protein, mouse
0
Histones
0
Intracellular Signaling Peptides and Proteins
0
Aurkb protein, mouse
EC 2.7.11.1
Aurora Kinase B
EC 2.7.11.1
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19901Informations de copyright
© 2021. The Author(s).
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