ZFP423 controls EBF2 coactivator recruitment and PPARγ occupancy to determine the thermogenic plasticity of adipocytes.
EBF2
PPARγ
ZFP423
beige adipocytes
rosiglitazone
white adipocytes
Journal
Genes & development
ISSN: 1549-5477
Titre abrégé: Genes Dev
Pays: United States
ID NLM: 8711660
Informations de publication
Date de publication:
01 11 2021
01 11 2021
Historique:
received:
26
06
2021
accepted:
07
09
2021
pubmed:
9
10
2021
medline:
5
4
2022
entrez:
8
10
2021
Statut:
ppublish
Résumé
Energy-storing white adipocytes maintain their identity by suppressing the energy-burning thermogenic gene program of brown and beige adipocytes. Here, we reveal that the protein-protein interaction between the transcriptional coregulator ZFP423 and brown fat determination factor EBF2 is essential for restraining the thermogenic phenotype of white adipose tissue (WAT). Disruption of the ZFP423-EBF2 protein interaction through CRISPR-Cas9 gene editing triggers widespread "browning" of WAT in adult mice. Mechanistically, ZFP423 recruits the NuRD corepressor complex to EBF2-bound thermogenic gene enhancers. Loss of adipocyte
Identifiants
pubmed: 34620682
pii: gad.348780.121
doi: 10.1101/gad.348780.121
pmc: PMC8559675
doi:
Substances chimiques
Basic Helix-Loop-Helix Transcription Factors
0
DNA-Binding Proteins
0
Ebf2 protein, mouse
0
Ebfaz protein, mouse
0
PPAR gamma
0
Pparg protein, mouse
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1461-1474Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK119163
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK121801
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK123356
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK104789
Pays : United States
Organisme : NIDDK NIH HHS
ID : RC2 DK118620
Pays : United States
Informations de copyright
© 2021 Shao et al.; Published by Cold Spring Harbor Laboratory Press.
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