Simple workflow to repurpose SARS-CoV-2 swab/serum samples for the isolation of cost-effective antibody/antigens for proteotyping applications and diagnosis.
Antigen/antibody purification
Lateral flow assay
Magnetic nanoparticle (MNP)–based separation
Mass spectrometry
SARS-CoV-2
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
07
08
2021
accepted:
07
09
2021
revised:
02
09
2021
pubmed:
9
10
2021
medline:
23
11
2021
entrez:
8
10
2021
Statut:
ppublish
Résumé
Supply shortage for the development and production of preventive, therapeutic, and diagnosis tools during the COVID-19 pandemic is an important issue affecting the wealthy and poor nations alike. Antibodies and antigens are especially needed for the production of immunological-based testing tools such as point-of-care tests. Here, we propose a simple and quick magnetic nanoparticle (MNP)-based separation/isolation approach for the repurposing of infected human samples to produce specific antibodies and antigen cocktails. Initially, an antibody cocktail was purified from serums via precipitation and immunoaffinity chromatography. Purified antibodies were conjugated onto MNPs and used as an affinity matrix to separate antigens. The characterization process was performed by ELISA, SDS-PAGE, electrochemistry, isothermal titration calorimetry, and LC-Q-TOF-MS/MS analyses. The MNP-separated peptides can be used for mass spectrometry-based as well as paper-based lateral flow assay diagnostic. The exploitation of the current workflow for the development of efficient diagnostic tools, specific treatments, and fundamental research can significantly impact the present or eventual pandemic. This workflow can be considered as a two birds, one stone-like strategy.
Identifiants
pubmed: 34622322
doi: 10.1007/s00216-021-03654-4
pii: 10.1007/s00216-021-03654-4
pmc: PMC8497067
doi:
Substances chimiques
Antibodies, Viral
0
Antigens, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7251-7263Subventions
Organisme : Türkiye Bilimsel ve Teknolojik Araştirma Kurumu
ID : 120R021
Organisme : Kalkinma Bakanliği
ID : 2016K121190
Informations de copyright
© 2021. Springer-Verlag GmbH Germany, part of Springer Nature.
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