Novel DNA Aptameric Sensors to Detect the Toxic Insecticide Fenitrothion.
SELEX
aptamer
fenitrothion
fluorescence
insecticide
thioflavin T
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
07 Oct 2021
07 Oct 2021
Historique:
received:
06
09
2021
revised:
29
09
2021
accepted:
05
10
2021
entrez:
13
10
2021
pubmed:
14
10
2021
medline:
26
10
2021
Statut:
epublish
Résumé
Fenitrothion is an insecticide belonging to the organophosphate family of pesticides that is widely used around the world in agriculture and living environments. Today, it is one of the most hazardous chemicals that causes severe environmental pollution. However, detection of fenitrothion residues in the environment is considered a significant challenge due to the small molecule nature of the insecticide and lack of molecular recognition elements that can detect it with high specificity. We performed in vitro selection experiments using the SELEX process to isolate the DNA aptamers that can bind to fenitrothion. We found that newly discovered DNA aptamers have a strong ability to distinguish fenitrothion from other organophosphate insecticides (non-specific targets). Furthermore, we identified a fenitrothion-specific aptamer; FenA2, that can interact with Thioflavin T (ThT) to produce a label-free detection mode with a
Identifiants
pubmed: 34639187
pii: ijms221910846
doi: 10.3390/ijms221910846
pmc: PMC8509669
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Insecticides
0
Plant Extracts
0
Fenitrothion
W8M4X3Y7ZY
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Rural Development Administration
ID : PJ01623501
Organisme : National Research Foundation of Korea
ID : 2020R1A1A03044344
Organisme : BK21 Program
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