Optimization of long-term cold storage of rat precision-cut lung slices with a tissue preservation solution.
3R
PCLS
cold storage
tissue preservation
Journal
American journal of physiology. Lung cellular and molecular physiology
ISSN: 1522-1504
Titre abrégé: Am J Physiol Lung Cell Mol Physiol
Pays: United States
ID NLM: 100901229
Informations de publication
Date de publication:
01 12 2021
01 12 2021
Historique:
pubmed:
14
10
2021
medline:
15
12
2021
entrez:
13
10
2021
Statut:
ppublish
Résumé
Precision-cut lung slices (PCLS) are used as ex vivo model of the lung to fill the gap between in vitro and in vivo experiments. To allow optimal utilization of PCLS, possibilities to prolong slice viability via cold storage using optimized storage solutions were evaluated. Rat PCLS were cold stored in DMEM/F-12 or two different preservation solutions for up to 28 days at 4°C. After rewarming in DMEM/F-12, metabolic activity, live/dead staining, and mitochondrial membrane potential was assessed to analyze overall tissue viability. Single-cell suspensions were prepared and proportions of CD45
Identifiants
pubmed: 34643087
doi: 10.1152/ajplung.00076.2021
doi:
Substances chimiques
Organ Preservation Solutions
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM