A transferrable IncL/M plasmid harboring a gene encoding IMP-1 metallo-β-lactamase in clinical isolates of Enterobacteriaceae.
Carbapenemase producing Enterobacteriaceae
IMP-1 metallo-β-lactamase
IncL/M
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
13 Oct 2021
13 Oct 2021
Historique:
received:
10
12
2020
accepted:
04
10
2021
entrez:
14
10
2021
pubmed:
15
10
2021
medline:
16
10
2021
Statut:
epublish
Résumé
The worldwide spread of carbapenemase-producing Enterobacteriaceae (CPE) has reduced the clinical utility of carbapenems. Plasmids often play an important role in the spread of genes encoding drug-resistance factors, especially in the horizontal transfer of these genes among species of Enterobacteriaceae. This study describes a patient infected with three species of CPE carrying an identical transferrable IncL/M plasmid. Clinical isolates of CPE were collected at St. Luke's International Hospital, Tokyo, Japan, from 2015 to 2019. Three species of CPE isolates, Enterobacter cloacae, Klebsiella aerogenes and Serratia marcescens, were isolated from a patient who developed severe gallstone pancreatitis associated with bloodstream infection, with all three isolates producing IMP-1 metallo-β-lactamase. The complete sequences of the plasmids of the three isolates were determined by both MiSeq and MinION. The medical chart of this patient was retrospectively reviewed conducted to obtain relevant clinical information. The three CPE species carried an IncL/M plasmid, pSL264, which was 81,133 bp in size and harbored bla The IncL/M plasmid pSL264 harboring bla
Sections du résumé
BACKGROUND
BACKGROUND
The worldwide spread of carbapenemase-producing Enterobacteriaceae (CPE) has reduced the clinical utility of carbapenems. Plasmids often play an important role in the spread of genes encoding drug-resistance factors, especially in the horizontal transfer of these genes among species of Enterobacteriaceae. This study describes a patient infected with three species of CPE carrying an identical transferrable IncL/M plasmid.
METHODS
METHODS
Clinical isolates of CPE were collected at St. Luke's International Hospital, Tokyo, Japan, from 2015 to 2019. Three species of CPE isolates, Enterobacter cloacae, Klebsiella aerogenes and Serratia marcescens, were isolated from a patient who developed severe gallstone pancreatitis associated with bloodstream infection, with all three isolates producing IMP-1 metallo-β-lactamase. The complete sequences of the plasmids of the three isolates were determined by both MiSeq and MinION. The medical chart of this patient was retrospectively reviewed conducted to obtain relevant clinical information.
RESULTS
RESULTS
The three CPE species carried an IncL/M plasmid, pSL264, which was 81,133 bp in size and harbored bla
CONCLUSION
CONCLUSIONS
The IncL/M plasmid pSL264 harboring bla
Identifiants
pubmed: 34645409
doi: 10.1186/s12879-021-06758-5
pii: 10.1186/s12879-021-06758-5
pmc: PMC8513173
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
beta-Lactamases
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1061Subventions
Organisme : japan agency for medical research and development
ID : 21fk0108604h0701
Informations de copyright
© 2021. The Author(s).
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