Arabidopsis thaliana myosin XIK is recruited to the Golgi through interaction with a MyoB receptor.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
13 10 2021
13 10 2021
Historique:
received:
16
07
2020
accepted:
31
08
2021
entrez:
14
10
2021
pubmed:
15
10
2021
medline:
24
12
2021
Statut:
epublish
Résumé
Plant cell organelles are highly mobile and their positioning play key roles in plant growth, development and responses to changing environmental conditions. Movement is acto-myosin dependent. Despite controlling the dynamics of several organelles, myosin and myosin receptors identified so far in Arabidopsis thaliana generally do not localise to the organelles whose movement they control, raising the issue of how specificity is determined. Here we show that a MyoB myosin receptor, MRF7, specifically localises to the Golgi membrane and affects its movement. Myosin XI-K was identified as a putative MRF7 interactor through mass spectrometry analysis. Co-expression of MRF7 and XI-K tail triggers the relocation of XI-K to the Golgi, linking a MyoB/myosin complex to a specific organelle in Arabidopsis. FRET-FLIM confirmed the in vivo interaction between MRF7 and XI-K tail on the Golgi and in the cytosol, suggesting that myosin/myosin receptor complexes perhaps cycle on and off organelle membranes. This work supports a traditional mechanism for organelle movement where myosins bind to receptors and adaptors on the organelle membranes, allowing them to actively move on the actin cytoskeleton, rather than passively in the recently proposed cytoplasmic streaming model.
Identifiants
pubmed: 34645991
doi: 10.1038/s42003-021-02700-2
pii: 10.1038/s42003-021-02700-2
pmc: PMC8514473
doi:
Substances chimiques
AT2G24140 protein, Arabidopsis
0
AT5G20490 protein, Arabidopsis
0
Arabidopsis Proteins
0
Membrane Proteins
0
Myosins
EC 3.6.4.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1182Subventions
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/F011709/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/I006184/2
Organisme : RCUK | STFC | Central Laser Facility, Science and Technology Facilities Council (Central Laser Facility at the STFC Rutherford Appleton Laboratory)
ID : PM-1216
Informations de copyright
© 2021. The Author(s).
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