A Multiplexed, Time-Resolved Assay of Root Gravitropic Bending on Agar Plates.
Arabidopsis thaliana
Automated scanning
Root gravitropism
Time-dependent change
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
14
10
2021
pubmed:
15
10
2021
medline:
11
1
2022
Statut:
ppublish
Résumé
The ability of roots to orient their growth relative to the vector of gravity, root gravitropism (positive gravitropism), is observed in root systems of higher plants and is an essential part of plant growth and development. While there are various methods for quantifying root gravitropism, many methods that can efficiently measure gravitropism at a reasonable throughput do not yield temporal resolution of the process, while methods that allow for high-temporal resolution are often not suitable for an efficient measurement of multiple roots. Here, we describe a method to analyze the root gravitropism activity at an increased throughput with a fine time-resolution using Arabidopsis thaliana plants.
Identifiants
pubmed: 34647248
doi: 10.1007/978-1-0716-1677-2_4
doi:
Substances chimiques
Arabidopsis Proteins
0
Agar
9002-18-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
61-70Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
Références
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