Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies.
Gravitropism
Polyethersulfone
Spaceflight
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
14
10
2021
pubmed:
15
10
2021
medline:
11
1
2022
Statut:
ppublish
Résumé
Polyethersulfone (PES) membranes provide a versatile tool for gravity-related plant studies. Benefits of this system include straightforward setup, no need for specialized equipment, long-term seed viability between plating and hydration/growth, and adaptability to diverse protocols and downstream analyses. Methods outlined here include seed sterilization, planting, growth, and dissection that will transition directly into any RNA extraction protocol.
Identifiants
pubmed: 34647259
doi: 10.1007/978-1-0716-1677-2_15
doi:
Substances chimiques
Arabidopsis Proteins
0
Polymers
0
Sulfones
0
polyether sulfone
25667-42-9
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
233-239Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
Références
Vandenbrink JP, Herranz R, Poehlman WL, Alex Feltus F, Villacampa A, Ciska M et al (2019) RNA-seq analyses of Arabidopsis thaliana seedlings after exposure to blue-light phototropic stimuli in microgravity. Am J Bot 106:1466–1476
doi: 10.1002/ajb2.1384
Correll MJ, Edelmann RE, Hangarter RP, Mullen JL, Kiss JZ (2005) Ground-based studies of tropisms in hardware developed for the European Modular Cultivation System (EMCS). Adv Space Res 36:1203–1210
doi: 10.1016/j.asr.2004.11.003