Flexible linker modulates the binding affinity of the TP901-1 CI phage repressor to DNA.
bacteriophage
flexible linker
genetic switch
lysogeny
repressor
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
revised:
16
09
2021
received:
29
06
2021
accepted:
18
10
2021
pubmed:
20
10
2021
medline:
3
3
2022
entrez:
19
10
2021
Statut:
ppublish
Résumé
Temperate bacteriophages can switch between two life cycles following infection of a host bacterium: the lytic or lysogenic life cycle. The choice between these is controlled by a bistable genetic switch. We investigated the genetic switch of the lactococcal temperate bacteriophage, TP901-1, which is controlled by two regulatory proteins, the Clear 1 (CI) repressor and modulator of repression (MOR) antirepressor. CI consists of a DNA-binding N-terminal domain and a C-terminal domain responsible for oligomerization, connected by a flexible interdomain linker. Full-length CI is hexameric, whereas the truncated version CI with 58 C-terminal residues truncated (CIΔ58), missing the second C-terminal subdomain, is dimeric, but binds with the same affinity as full-length CI to the O
Substances chimiques
Repressor Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1135-1148Informations de copyright
© 2021 Federation of European Biochemical Societies.
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