A rapid assay provides on-site quantification of tetrahydrocannabinol in oral fluid.
Journal
Science translational medicine
ISSN: 1946-6242
Titre abrégé: Sci Transl Med
Pays: United States
ID NLM: 101505086
Informations de publication
Date de publication:
20 Oct 2021
20 Oct 2021
Historique:
entrez:
20
10
2021
pubmed:
21
10
2021
medline:
3
11
2021
Statut:
ppublish
Résumé
Tetrahydrocannabinol (THC), the primary psychoactive ingredient of cannabis, impairs cognitive and motor function in a concentration-dependent fashion. Drug testing is commonly performed for employment and law enforcement purposes; however, available tests produce low-sensitive binary results (lateral flow assays) or have long turnaround (gas chromatography–mass spectrometry). To enable on-site THC quantification in minutes, we developed a rapid assay for oral THC analysis called EPOCH (express probe for on-site cannabis inhalation). EPOCH features distinctive sensor design such as a radial membrane and transmission optics, all contained in a compact cartridge. This integrated approach permitted assay completion within 5 min with a detection limit of 0.17 ng/ml THC, which is below the regulatory guideline (1 ng/ml). As a proof of concept for field testing, we applied EPOCH to assess oral fluid samples from cannabis users (
Identifiants
pubmed: 34669441
doi: 10.1126/scitranslmed.abe2352
pmc: PMC9126021
mid: NIHMS1804840
doi:
Substances chimiques
Dronabinol
7J8897W37S
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
eabe2352Subventions
Organisme : NCI NIH HHS
ID : R01 CA239078
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA229777
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA264363
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA233360
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA237500
Pays : United States
Organisme : NIDA NIH HHS
ID : R21 DA049577
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA204019
Pays : United States
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