The role of estrogen and progesterone receptors in the rotator cuff disease: a retrospective cohort study.
Cuff
Estrogen
Immunohistochemistry
Progesterone
Receptor
Rotator
Tendinopathy
Journal
BMC musculoskeletal disorders
ISSN: 1471-2474
Titre abrégé: BMC Musculoskelet Disord
Pays: England
ID NLM: 100968565
Informations de publication
Date de publication:
20 Oct 2021
20 Oct 2021
Historique:
received:
17
04
2021
accepted:
22
09
2021
entrez:
21
10
2021
pubmed:
22
10
2021
medline:
27
10
2021
Statut:
epublish
Résumé
Rotator cuff (RC) tears represent a common cause of shoulder pain and dysfunction in adults. The disease affects primarily women and occurs mainly in the postmenopausal period. This study aimed to investigate immunohistochemically the presence of estrogen receptor-alpha (ER-⍺), estrogen receptor-beta (ER-β) and progesterone receptor (PR) in the supraspinatus tendon of patients with RC tendinopathy, searching for gender differences of expression. A secondary aim was to evaluate potential links between their expression and the typical histopathological findings of the ailment. Biopsies of the supraspinatus tendon were collected intraoperatively from 15 postmenopausal women and 9 men undergoing RC surgery. Specimens were stained with Haematoxylin/Eosin, Masson-Goldner Trichrome, Alcian Blu and immunohistochemical stainings for ER-⍺, ER-β and PR were performed. Tendon alterations were evaluated with the Bonar histopathological scale. Statistical tests used in this study were the Spearman correlation coefficient and the Mann-Whitney U test. In the supraspinatus tendon, cells expressed ER-⍺ (p = 0.043), ER-β (p = 0.048) and PR (p = 0.004) with statistically significant differences related to age and sex of patients. Immunoreactivity was seen in the nuclei of tenocytes and vascular cells. Postmenopausal women's samples showed a markedly higher expression of these receptors compared to their male counterpart. There was a positive correlation between the expression of ER-⍺ and ER-β (r = 0.59; p = 0.02) and between ER-β and PR (r = 0.72; p = 0.002) in women's samples. Furthermore, in postmenopausal women the PR expression decreased with age (r = - 0.56; p = 0.027). Only in women, the ER-β expression positively correlated with the total Bonar histopathological score (p = 0.019) and the ER-β vascular expression positively correlated with ground substance alterations (p = 0.029). These results reveal that ERs and PR are present in the supraspinatus tendon of patients with RC tears, suggesting a role of sex hormones in the pathogenesis of the disease.
Sections du résumé
BACKGROUND
BACKGROUND
Rotator cuff (RC) tears represent a common cause of shoulder pain and dysfunction in adults. The disease affects primarily women and occurs mainly in the postmenopausal period. This study aimed to investigate immunohistochemically the presence of estrogen receptor-alpha (ER-⍺), estrogen receptor-beta (ER-β) and progesterone receptor (PR) in the supraspinatus tendon of patients with RC tendinopathy, searching for gender differences of expression. A secondary aim was to evaluate potential links between their expression and the typical histopathological findings of the ailment.
METHODS
METHODS
Biopsies of the supraspinatus tendon were collected intraoperatively from 15 postmenopausal women and 9 men undergoing RC surgery. Specimens were stained with Haematoxylin/Eosin, Masson-Goldner Trichrome, Alcian Blu and immunohistochemical stainings for ER-⍺, ER-β and PR were performed. Tendon alterations were evaluated with the Bonar histopathological scale. Statistical tests used in this study were the Spearman correlation coefficient and the Mann-Whitney U test.
RESULTS
RESULTS
In the supraspinatus tendon, cells expressed ER-⍺ (p = 0.043), ER-β (p = 0.048) and PR (p = 0.004) with statistically significant differences related to age and sex of patients. Immunoreactivity was seen in the nuclei of tenocytes and vascular cells. Postmenopausal women's samples showed a markedly higher expression of these receptors compared to their male counterpart. There was a positive correlation between the expression of ER-⍺ and ER-β (r = 0.59; p = 0.02) and between ER-β and PR (r = 0.72; p = 0.002) in women's samples. Furthermore, in postmenopausal women the PR expression decreased with age (r = - 0.56; p = 0.027). Only in women, the ER-β expression positively correlated with the total Bonar histopathological score (p = 0.019) and the ER-β vascular expression positively correlated with ground substance alterations (p = 0.029).
CONCLUSIONS
CONCLUSIONS
These results reveal that ERs and PR are present in the supraspinatus tendon of patients with RC tears, suggesting a role of sex hormones in the pathogenesis of the disease.
Identifiants
pubmed: 34670550
doi: 10.1186/s12891-021-04778-5
pii: 10.1186/s12891-021-04778-5
pmc: PMC8529750
doi:
Substances chimiques
Estrogens
0
Receptors, Estrogen
0
Receptors, Progesterone
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
891Informations de copyright
© 2021. The Author(s).
Références
Nat Rev Cancer. 2017 Jan;17(1):54-64
pubmed: 27885264
J Shoulder Elbow Surg. 2014 Feb;23(2):227-35
pubmed: 24129055
Med Sport Sci. 2012;57:1-9
pubmed: 21986040
Calcif Tissue Int. 1993 Nov;53(5):312-7
pubmed: 8287318
J Shoulder Elbow Surg. 2012 Jan;21(1):56-60
pubmed: 21524922
Steroids. 2001 Jan;66(1):1-16
pubmed: 11090653
Genes Dev. 2018 Sep 1;32(17-18):1141-1154
pubmed: 30181360
Acta Orthop Scand. 1976 Oct;47(5):473-9
pubmed: 998180
Cell Biol Int. 2008 Jan;32(1):75-9
pubmed: 17951080
Int J Mol Sci. 2015 Nov 04;16(11):26452-62
pubmed: 26556342
Ann Clin Biochem. 2001 Sep;38(Pt 5):527-32
pubmed: 11587131
J Shoulder Elbow Surg. 2010 Jan;19(1):116-20
pubmed: 19540777
Am J Sports Med. 2008 Mar;36(3):533-8
pubmed: 18006676
J Anat. 2000 Nov;197 Pt 4:617-25
pubmed: 11197535
Clin Orthop Relat Res. 1976 Jul-Aug;(118):164-8
pubmed: 954272
Muscles Ligaments Tendons J. 2014 Nov 17;4(3):309-14
pubmed: 25489548
Am J Obstet Gynecol. 2004 Mar;190(3):620-7
pubmed: 15041990
J Histochem Cytochem. 1986 May;34(5):599-605
pubmed: 3517144
J Appl Physiol (1985). 2001 Oct;91(4):1860-7
pubmed: 11568173
J Orthop Res. 2004 Mar;22(2):334-8
pubmed: 15013093
Physiol Rev. 2007 Jul;87(3):905-31
pubmed: 17615392
Sports Med Arthrosc Rev. 2011 Sep;19(3):227-36
pubmed: 21822106
Ann Intern Med. 1994 Dec 15;121(12):936-41
pubmed: 7978718
PLoS Med. 2010 May 25;7(5):e1000279
pubmed: 20520800
PLoS One. 2018 Oct 8;13(10):e0204603
pubmed: 30296306
J Appl Physiol (1985). 2009 Apr;106(4):1385-93
pubmed: 18927264
Age (Dordr). 2013 Dec;35(6):2125-36
pubmed: 23274854
Am J Sports Med. 2008 Jan;36(1):99-103
pubmed: 17932406
Biochem Biophys Res Commun. 2004 May 14;317(4):1221-5
pubmed: 15094400
J Orthop Sports Phys Ther. 2014 Mar;44(3):153-63
pubmed: 24450368
Knee Surg Sports Traumatol Arthrosc. 2007 Dec;15(12):1482-5
pubmed: 17497128
Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7821-6
pubmed: 18509053
Curr Rev Musculoskelet Med. 2018 Mar;11(1):77-85
pubmed: 29411321
Clin Sports Med. 2012 Oct;31(4):589-604
pubmed: 23040548
Endocrinology. 1993 Aug;133(2):829-37
pubmed: 8344219
Am J Sports Med. 2000 Nov-Dec;28(6):857-63
pubmed: 11101109
J Shoulder Elbow Surg. 2015 Feb;24(2):e31-5
pubmed: 25219474
Clin Orthop Relat Res. 1998 Jun;(351):44-56
pubmed: 9646746
Clin Orthop Relat Res. 2001 Feb;(383):268-81
pubmed: 11210964
Muscles Ligaments Tendons J. 2014 Nov 17;4(3):324-8
pubmed: 25489550
Br J Cancer. 2014 Feb 4;110(3):565-72
pubmed: 24300977
Biochem Biophys Res Commun. 2005 Mar 18;328(3):688-96
pubmed: 15694402
J Orthop Res. 1996 Jul;14(4):526-33
pubmed: 8764860
Br Med Bull. 2019 Jun 19;130(1):105-123
pubmed: 31093655
Br Med Bull. 2016 Mar;117(1):39-58
pubmed: 26790696
J Bone Joint Surg Am. 2014 May 21;96(10):793-800
pubmed: 24875019
Oper Tech Orthop. 2015 Mar 1;25(1):2-9
pubmed: 26726288
Connect Tissue Res. 1979;7(1):21-7
pubmed: 158483
J Steroid Biochem Mol Biol. 2017 Sep;172:106-116
pubmed: 28629994
Am J Physiol. 1977 Jun;232(6):H617-21
pubmed: 879300
Arch Orthop Trauma Surg. 2017 Feb;137(2):217-224
pubmed: 27933383
Curr Opin Cardiol. 1994 Sep;9(5):619-26
pubmed: 7987043
Menopause. 2014 Mar;21(3):275-80
pubmed: 23760436
J Bone Joint Surg Br. 2004 Jul;86(5):696-700
pubmed: 15274266
Int J Oral Maxillofac Surg. 2016 Mar;45(3):323-31
pubmed: 26584852
EMBO J. 2012 Nov 14;31(22):4255-7
pubmed: 23064149
Endocrinology. 1983 Dec;113(6):2195-201
pubmed: 6685620
J Shoulder Elbow Surg. 1999 Jul-Aug;8(4):296-9
pubmed: 10471998
Endocrinology. 1973 Apr;92(4):1229-40
pubmed: 4686319
J Orthop Res. 2010 Nov;28(11):1469-74
pubmed: 20872583
J Shoulder Elbow Surg. 2016 Jun;25(6):865-72
pubmed: 27066960
Clin Exp Rheumatol. 2000 Nov-Dec;18(6):743-5
pubmed: 11138340
BMC Musculoskelet Disord. 2007 Dec 18;8:123
pubmed: 18088422