Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold.
Animals
Bone Regeneration
/ drug effects
Cell Adhesion Molecules
/ metabolism
Collagen
/ administration & dosage
Collagen Type I
/ metabolism
Dental Cementum
/ chemistry
Dogs
Guided Tissue Regeneration, Periodontal
Integrin-Binding Sialoprotein
/ metabolism
Keratins
/ metabolism
Periodontal Debridement
Periodontal Ligament
/ chemistry
Porosity
Proliferating Cell Nuclear Antigen
/ metabolism
Tissue Scaffolds
/ chemistry
biomaterial
collagen scaffold
histology
immunohistochemistry
intrabony defect
periodontal regeneration
volume-stable collagen matrix
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
09 Oct 2021
09 Oct 2021
Historique:
received:
22
09
2021
revised:
03
10
2021
accepted:
07
10
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
15
12
2021
Statut:
epublish
Résumé
(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%,
Identifiants
pubmed: 34681574
pii: ijms222010915
doi: 10.3390/ijms222010915
pmc: PMC8535773
pii:
doi:
Substances chimiques
Cell Adhesion Molecules
0
Collagen Type I
0
Integrin-Binding Sialoprotein
0
Proliferating Cell Nuclear Antigen
0
Keratins
68238-35-7
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Geistlich Pharma (Switzerland)
ID : 300409-101
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