Cyclic FEE Peptide Improves Human Sperm Movement Parameters without Modification of Their Energy Metabolism.
Adenosine Triphosphate
/ metabolism
Energy Metabolism
Humans
Luminescent Measurements
Male
Membrane Potential, Mitochondrial
/ drug effects
Membranes
/ metabolism
Mitochondria
/ drug effects
Oxidative Phosphorylation
Peptides, Cyclic
/ pharmacology
Sperm Motility
/ drug effects
Spermatozoa
/ drug effects
ATP
fertilization
motility
spermatozoa
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
19 Oct 2021
19 Oct 2021
Historique:
received:
30
09
2021
revised:
14
10
2021
accepted:
15
10
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Cyclic fertilin peptide (cFEE: phenylalanine, glutamic acid; glutamic acid) improves gamete interaction in humans. We investigate whether it could be via improvement of sperm movement parameters and their mitochondrial ATP production. Sperm movement parameters were studied using computer-assisted sperm analysis (CASA) in sperm samples from 38 patients with normal sperm in medium supplemented with cyclic fertilin against a control group. Sperm mitochondrial functions were studied using donor's sperm, incubated or not with cFEE. It was evaluated by the measurement of their ATP production using bioluminescence, their respiration by high resolution oxygraphy, and of mitochondrial membrane potential (MMP) using potentiometric dyes and flow cytometry. cFEE significantly improved sperm movement parameters and percentage of hyperactivated sperm. Impact of inhibitors showed OXPHOS as the predominant energy source for sperm movement. However, cFEE had no significant impact on any of the analyzed mitochondrial bioenergetic parameters, suggesting that it could act via a more efficient use of its energy resources.
Identifiants
pubmed: 34681924
pii: ijms222011263
doi: 10.3390/ijms222011263
pmc: PMC8539654
pii:
doi:
Substances chimiques
Peptides, Cyclic
0
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-17-CE18-0005-02
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