DREAM represses distinct targets by cooperating with different THAP domain proteins.
Animals
Animals, Genetically Modified
Caenorhabditis elegans
/ genetics
Caenorhabditis elegans Proteins
/ genetics
Cell Cycle Proteins
/ genetics
DNA Methylation
E2F Transcription Factors
/ genetics
Gene Expression Regulation
Histones
/ genetics
Promoter Regions, Genetic
Protein Binding
Protein Interaction Domains and Motifs
Retinoblastoma Protein
/ genetics
Transcription Factors
/ genetics
DREAM
H2A.Z
H3K9me2
THAP
lin-15B
lin-35
lin-36
quiescence
retinoblastoma
transcriptional repression
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
19 10 2021
19 10 2021
Historique:
received:
28
08
2020
revised:
03
06
2021
accepted:
24
09
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
16
2
2022
Statut:
ppublish
Résumé
The DREAM (dimerization partner [DP], retinoblastoma [Rb]-like, E2F, and MuvB) complex controls cellular quiescence by repressing cell-cycle and other genes, but its mechanism of action is unclear. Here, we demonstrate that two C. elegans THAP domain proteins, LIN-15B and LIN-36, co-localize with DREAM and function by different mechanisms for repression of distinct sets of targets. LIN-36 represses classical cell-cycle targets by promoting DREAM binding and gene body enrichment of H2A.Z, and we find that DREAM subunit EFL-1/E2F is specific for LIN-36 targets. In contrast, LIN-15B represses germline-specific targets in the soma by facilitating H3K9me2 promoter marking. We further find that LIN-36 and LIN-15B differently regulate DREAM binding. In humans, THAP proteins have been implicated in cell-cycle regulation by poorly understood mechanisms. We propose that THAP domain proteins are key mediators of Rb/DREAM function.
Identifiants
pubmed: 34686342
pii: S2211-1247(21)01299-7
doi: 10.1016/j.celrep.2021.109835
pmc: PMC8552245
pii:
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Cell Cycle Proteins
0
E2F Transcription Factors
0
Histones
0
Retinoblastoma Protein
0
Transcription Factors
0
efl-1 protein, C elegans
0
lin-15B protein, C elegans
0
lin-36 protein, C elegans
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
109835Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 101863
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S021620/1
Pays : United Kingdom
Organisme : Wellcome
ID : 092096
Organisme : Cancer Research UK
ID : C6946/A14492
Pays : United Kingdom
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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