Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents.
COVID-19
Contamination
Cross-well
Hamilton NIMBUS
Magnetic beads
Nucleic acid extraction
RNA
SARS-CoV-2
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
18
05
2021
revised:
06
10
2021
accepted:
13
10
2021
pubmed:
24
10
2021
medline:
15
12
2021
entrez:
23
10
2021
Statut:
ppublish
Résumé
The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.
Identifiants
pubmed: 34687784
pii: S0166-0934(21)00278-0
doi: 10.1016/j.jviromet.2021.114339
pmc: PMC8527638
pii:
doi:
Substances chimiques
Indicators and Reagents
0
Nucleic Acids
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
114339Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
Références
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