EDTA stabilizes the concentration of platelet-derived extracellular vesicles during blood collection and handling.

Anticoagulants / pharmacology Blood Platelets Citrates / pharmacology Citric Acid / pharmacology Edetic Acid / pharmacology Extracellular Vesicles Humans Platelet Activation
Anticoagulants biomarkers blood platelets extracellular vesicles flow cytometry

Journal

Platelets
ISSN: 1369-1635
Titre abrégé: Platelets
Pays: England
ID NLM: 9208117

Informations de publication

Date de publication:
04 Jul 2022
Historique:
pubmed: 27 10 2021
medline: 31 5 2022
entrez: 26 10 2021
Statut: ppublish

Résumé

Citrate is the recommended anticoagulant for studies on plasma extracellular vesicles (EVs). Because citrate incompletely blocks platelet activation and the release of platelet-derived EVs, we compared EDTA and citrate in that regard. Blood from healthy individuals (n = 7) was collected and incubated with thrombin receptor-activating peptide-6 (TRAP-6) to activate platelets, subjected to pneumatic tube transportation (n = 6), a freeze-thaw cycle (n = 10), and stored before plasma preparation (n = 6). Concentrations of EVs from platelets (CD61

Identifiants

DOI: 10.1080/09537104.2021.1991569 PMID: 34697987
pubmed: 34697987
doi: 10.1080/09537104.2021.1991569
doi:

Substances chimiques

Anticoagulants 0
Citrates 0
Citric Acid 2968PHW8QP
Edetic Acid 9G34HU7RV0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

764-771

Auteurs

Naomi C Buntsma (NC)

Department of Neurology, Amsterdam UMC, University of Amsterdam, The Netherlands.
Biomedical Engineering & Physics, Amsterdam UMC, University of Amsterdam, The Netherlands.
Vesicle Observation Centre, and Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, The Netherlands.
ORCID: 0000-0003-3201-7205

Aleksandra Gąsecka (A)

Vesicle Observation Centre, and Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, The Netherlands.
1st Chair and Department of Cardiology, Medical University of Warsaw, Warsaw Poland.
ORCID: 0000-0001-5083-7587

Yvo B W E M Roos (YBWEM)

Department of Neurology, Amsterdam UMC, University of Amsterdam, The Netherlands.
ORCID: 0000-0001-9205-5882

Ton G van Leeuwen (TG)

Biomedical Engineering & Physics, Amsterdam UMC, University of Amsterdam, The Netherlands.
ORCID: 0000-0002-5642-1133

Edwin van der Pol (E)

Biomedical Engineering & Physics, Amsterdam UMC, University of Amsterdam, The Netherlands.
Vesicle Observation Centre, and Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, The Netherlands.
ORCID: 0000-0002-9497-8426

Rienk Nieuwland (R)

Biomedical Engineering & Physics, Amsterdam UMC, University of Amsterdam, The Netherlands.
Vesicle Observation Centre, and Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, The Netherlands.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Agents hématologiques › Anticoagulants › EDTA stabilizes the concentration of...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Cellules sanguines › Plaquettes › EDTA stabilizes the concentration of...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Triacides carboxyliques › Citrates › EDTA stabilizes the concentration of...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Triacides carboxyliques › Citrates › Acide citrique › EDTA stabilizes the concentration of...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Acide édétique › EDTA stabilizes the concentration of...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Vésicules extracellulaires › EDTA stabilizes the concentration of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › EDTA stabilizes the concentration of...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiogiques du sang › Hémostase › Activation plaquettaire › EDTA stabilizes the concentration of...