Analysis of urinary kallikrein-related peptidase 13 for monitoring bladder cancer.
Bladder cancer
fluorogenic substrates
kallikrein-related peptidase 13
proteolytic activity
western blot
Journal
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals
ISSN: 1366-5804
Titre abrégé: Biomarkers
Pays: England
ID NLM: 9606000
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
pubmed:
28
10
2021
medline:
8
3
2022
entrez:
27
10
2021
Statut:
ppublish
Résumé
Bladder cancer (BC) is one of the 10 most common types of cancer worldwide, with approximately 550,000 new cases each year. Early detection and appropriate diagnosis are important factors in successful treatment of the disease. We used specific fluorogenic substrate for the quantitative determination of urine kallikrein 13 (KLK13) activity in healthy ( Urine samples from patients with G2 and G3 grade BC contained proteolytically active KLK13, as confirmed by kinetic analysis and immunochemical detection. KLK13 was not detected in the urine of patients with G1 grade BC. Previous clinical study reveals the KLK13 significance for BC prognosis as increased Using specific chromogenic/fluorogenic peptides could be useful for the non-invasive disease monitoring of BC progress.
Sections du résumé
BACKGROUND
BACKGROUND
Bladder cancer (BC) is one of the 10 most common types of cancer worldwide, with approximately 550,000 new cases each year. Early detection and appropriate diagnosis are important factors in successful treatment of the disease.
MATERIAL AND METHODS
METHODS
We used specific fluorogenic substrate for the quantitative determination of urine kallikrein 13 (KLK13) activity in healthy (
RESULTS
RESULTS
Urine samples from patients with G2 and G3 grade BC contained proteolytically active KLK13, as confirmed by kinetic analysis and immunochemical detection. KLK13 was not detected in the urine of patients with G1 grade BC.
DISCUSSION
CONCLUSIONS
Previous clinical study reveals the KLK13 significance for BC prognosis as increased
CONCLUSIONS
CONCLUSIONS
Using specific chromogenic/fluorogenic peptides could be useful for the non-invasive disease monitoring of BC progress.
Identifiants
pubmed: 34704886
doi: 10.1080/1354750X.2021.1999502
doi:
Substances chimiques
Biomarkers, Tumor
0
KLK13 protein, human
EC 3.4.21.-
Kallikreins
EC 3.4.21.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM