X-treme loss of sequence diversity linked to neo-X chromosomes in filarial nematodes.


Journal

PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488

Informations de publication

Date de publication:
10 2021
Historique:
received: 18 12 2020
accepted: 24 09 2021
revised: 08 11 2021
pubmed: 28 10 2021
medline: 17 12 2021
entrez: 27 10 2021
Statut: epublish

Résumé

The sequence diversity of natural and laboratory populations of Brugia pahangi and Brugia malayi was assessed with Illumina resequencing followed by mapping in order to identify single nucleotide variants and insertions/deletions. In natural and laboratory Brugia populations, there is a lack of sequence diversity on chromosome X relative to the autosomes (πX/πA = 0.2), which is lower than the expected (πX/πA = 0.75). A reduction in diversity is also observed in other filarial nematodes with neo-X chromosome fusions in the genera Onchocerca and Wuchereria, but not those without neo-X chromosome fusions in the genera Loa and Dirofilaria. In the species with neo-X chromosome fusions, chromosome X is abnormally large, containing a third of the genetic material such that a sizable portion of the genome is lacking sequence diversity. Such profound differences in genetic diversity can be consequential, having been associated with drug resistance and adaptability, with the potential to affect filarial eradication.

Identifiants

pubmed: 34705823
doi: 10.1371/journal.pntd.0009838
pii: PNTD-D-20-02198
pmc: PMC8575316
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0009838

Subventions

Organisme : NIAID NIH HHS
ID : U19 AI110820
Pays : United States

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

John Mattick (J)

Institute for Genome Science, University of Maryland, Baltimore, Maryland, United States of America.

Silvia Libro (S)

New England Biolabs, Ipswich, Massachusetts, United States of America.

Robin Bromley (R)

Institute for Genome Science, University of Maryland, Baltimore, Maryland, United States of America.

Wanpen Chaicumpa (W)

Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Matthew Chung (M)

Institute for Genome Science, University of Maryland, Baltimore, Maryland, United States of America.

Darren Cook (D)

Centre for Neglected Tropical Diseases, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.

Mohammad Behram Khan (MB)

Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Nikhil Kumar (N)

Institute for Genome Science, University of Maryland, Baltimore, Maryland, United States of America.

Yee-Ling Lau (YL)

Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Shailja Misra-Bhattacharya (S)

Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, India.

Ramakrishna Rao (R)

Division of Infectious Diseases, Washington University School of Medicine, St Louis, Missouri, United States of America.

Lisa Sadzewicz (L)

Institute for Genome Science, University of Maryland, Baltimore, Maryland, United States of America.

Atiporn Saeung (A)

Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

Mohd Shahab (M)

Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, India.

Benjamin C Sparklin (BC)

Institute for Genome Science, University of Maryland, Baltimore, Maryland, United States of America.

Andrew Steven (A)

Centre for Neglected Tropical Diseases, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.

Joseph D Turner (JD)

Centre for Neglected Tropical Diseases, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.

Luke J Tallon (LJ)

Institute for Genome Science, University of Maryland, Baltimore, Maryland, United States of America.

Mark J Taylor (MJ)

Centre for Neglected Tropical Diseases, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.

Andrew R Moorhead (AR)

Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.

Michelle Michalski (M)

University of Wisconsin Oshkosh, Oshkosh, Wisconsin, United States of America.

Jeremy M Foster (JM)

New England Biolabs, Ipswich, Massachusetts, United States of America.

Julie C Dunning Hotopp (JC)

Institute for Genome Science, University of Maryland, Baltimore, Maryland, United States of America.
Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland, United States of America.
Greenebaum Cancer Center, University of Maryland, Baltimore, Maryland, United States of America.

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