Targeting Oncogenic Src Homology 2 Domain-Containing Phosphatase 2 (SHP2) by Inhibiting Its Protein-Protein Interactions.


Journal

Journal of medicinal chemistry
ISSN: 1520-4804
Titre abrégé: J Med Chem
Pays: United States
ID NLM: 9716531

Informations de publication

Date de publication:
11 11 2021
Historique:
pubmed: 30 10 2021
medline: 1 2 2022
entrez: 29 10 2021
Statut: ppublish

Résumé

We developed a new class of inhibitors of protein-protein interactions of the SHP2 phosphatase, which is pivotal in cell signaling and represents a central target in the therapy of cancer and rare diseases. Currently available SHP2 inhibitors target the catalytic site or an allosteric pocket but lack specificity or are ineffective for disease-associated SHP2 mutants. Considering that pathogenic lesions cause signaling hyperactivation due to increased levels of SHP2 association with cognate proteins, we developed peptide-based molecules with nanomolar affinity for the N-terminal Src homology domain of SHP2, good selectivity, stability to degradation, and an affinity for pathogenic variants of SHP2 that is 2-20 times higher than for the wild-type protein. The best peptide reverted the effects of a pathogenic variant (D61G) in zebrafish embryos. Our results provide a novel route for SHP2-targeted therapies and a tool for investigating the role of protein-protein interactions in the function of SHP2.

Identifiants

pubmed: 34714648
doi: 10.1021/acs.jmedchem.1c01371
pmc: PMC8591604
doi:

Substances chimiques

Protein Tyrosine Phosphatase, Non-Receptor Type 11 EC 3.1.3.48

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

15973-15990

Références

J Med Chem. 2019 Feb 28;62(4):1781-1792
pubmed: 30688462
Sci Signal. 2012 Sep 25;5(243):ra68
pubmed: 23012655
Dev Dyn. 1995 Jul;203(3):253-310
pubmed: 8589427
Lancet. 2013 Jan 26;381(9863):333-42
pubmed: 23312968
Eur J Biochem. 2001 Jul;268(14):3898-906
pubmed: 11453982
J Biol Chem. 1997 Jun 27;272(26):16421-30
pubmed: 9195950
J Med Chem. 2016 Sep 8;59(17):7773-82
pubmed: 27347692
J Med Chem. 2007 Jul 26;50(15):3604-17
pubmed: 17580848
Mol Cell Proteomics. 2008 Apr;7(4):768-84
pubmed: 17956856
Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):14924-9
pubmed: 23980151
Nat Commun. 2018 Oct 30;9(1):4508
pubmed: 30375388
J Biol Chem. 1994 Apr 8;269(14):10467-74
pubmed: 8144631
Cell Mol Life Sci. 2006 Nov;63(22):2682-93
pubmed: 17075693
J Biol Chem. 1993 Oct 25;268(30):22771-6
pubmed: 8226787
Hum Mutat. 2020 Jun;41(6):1171-1182
pubmed: 32112654
Biochim Biophys Acta. 2005 Feb 14;1747(1):1-25
pubmed: 15680235
J Biol Chem. 2012 Aug 3;287(32):27066-77
pubmed: 22711529
J Phys Chem B. 2018 Dec 13;122(49):11108-11114
pubmed: 30047735
Nat Genet. 2003 Jun;34(2):148-50
pubmed: 12717436
J Mol Model. 2006 Feb;12(3):281-9
pubmed: 16240095
J Med Chem. 2020 Dec 10;63(23):14780-14804
pubmed: 33210922
J Chem Inf Model. 2020 Jun 22;60(6):3157-3171
pubmed: 32395997
Cold Spring Harb Perspect Biol. 2013 Dec 01;5(12):a008987
pubmed: 24296166
Mol Cell Biol. 1994 Oct;14(10):6674-82
pubmed: 7935386
J Exp Med. 2011 Sep 26;208(10):1977-88
pubmed: 21930766
PLoS Genet. 2007 Dec;3(12):e225
pubmed: 18159945
Mol Cell Biol. 1996 Dec;16(12):6887-99
pubmed: 8943344
Am J Hum Genet. 2002 Aug;71(2):389-94
pubmed: 12058348
Nature. 2016 Jul 7;535(7610):148-52
pubmed: 27362227
Biochemistry. 2009 Nov 3;48(43):10473-82
pubmed: 19795889
Nat Rev Drug Discov. 2018 Nov 28;17(12):847-849
pubmed: 30482950
J Med Chem. 2018 Feb 22;61(4):1382-1414
pubmed: 28737935
Proteins. 2007 Mar 1;66(4):963-74
pubmed: 17177198
J Biol Chem. 2001 Jun 29;276(26):24380-7
pubmed: 11323411
J Struct Biol X. 2020 May 27;4:100026
pubmed: 32647828
J Mol Biol. 1999 Nov 5;293(4):971-85
pubmed: 10543978
J Med Genet. 2002 Aug;39(8):571-4
pubmed: 12161596
Biochim Biophys Acta. 2007 Jun;1768(6):1550-8
pubmed: 17466938
J Med Chem. 2009 Mar 26;52(6):1612-8
pubmed: 19226165
Curr Top Med Chem. 2006;6(14):1465-71
pubmed: 16918462
Biochemistry. 1988 Mar 22;27(6):1839-43
pubmed: 3288278
Oncogene. 1999 Jun 24;18(25):3696-702
pubmed: 10391677
Hum Mol Genet. 2008 Jul 1;17(13):2018-29
pubmed: 18372317
Am J Hum Genet. 2006 Feb;78(2):279-90
pubmed: 16358218
Nat Genet. 2001 Dec;29(4):465-8
pubmed: 11704759
Sci Signal. 2019 Jan 08;12(563):
pubmed: 30622194
J Med Chem. 2020 Nov 25;63(22):13578-13594
pubmed: 32910655
Blood. 2004 Jul 15;104(2):307-13
pubmed: 14982869
Biochemistry. 1998 Jun 23;37(25):9083-90
pubmed: 9636054
J Med Chem. 2020 Oct 22;63(20):11368-11396
pubmed: 32460492
Bioorg Med Chem. 2020 Jun 15;28(12):115542
pubmed: 32503696
Sci Adv. 2020 Jan 31;6(5):eaay4458
pubmed: 32064351
Trends Cancer. 2018 Sep;4(9):616-633
pubmed: 30149880
Structure. 1994 May 15;2(5):423-38
pubmed: 7521735
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3199-203
pubmed: 7536927
Annu Rev Biophys Biomol Struct. 1997;26:259-88
pubmed: 9241420
J Biol Chem. 1994 May 6;269(18):13614-22
pubmed: 7513703
Mol Cell. 2003 Dec;12(6):1379-89
pubmed: 14690593
Biochemistry. 2014 Jul 1;53(25):4136-51
pubmed: 24935154
Cell. 1998 Feb 20;92(4):441-50
pubmed: 9491886
J Biol Chem. 1998 Feb 27;273(9):4904-8
pubmed: 9478933
Biochemistry. 1995 Jan 24;34(3):1040-9
pubmed: 7530043
Comput Struct Biotechnol J. 2021 Apr 20;19:2398-2415
pubmed: 34025932
Mol Cell Biol. 2002 Jun;22(12):4062-72
pubmed: 12024020
Org Biomol Chem. 2020 Jan 28;18(4):583-605
pubmed: 31777907
Science. 2002 Jan 25;295(5555):683-6
pubmed: 11743164
J Biol Chem. 2006 Jul 21;281(29):20271-82
pubmed: 16702225
J Biomol Screen. 2003 Feb;8(1):34-8
pubmed: 12854996
Nature. 1995 Feb 16;373(6515):573-80
pubmed: 7531822
J Biol Chem. 1998 Apr 10;273(15):9234-42
pubmed: 9535915
Eur J Med Genet. 2015 Oct;58(10):509-25
pubmed: 26341048
Mol Pharm. 2013 May 6;10(5):2008-20
pubmed: 23537165
Nat Struct Biol. 1998 Feb;5(2):156-63
pubmed: 9461082
Cell Rep. 2015 Sep 29;12(12):1978-85
pubmed: 26365186
FEBS Open Bio. 2018 Aug 01;8(9):1405-1411
pubmed: 30186742
Biochemistry. 2002 Jul 23;41(29):9229-36
pubmed: 12119038
J Biol Chem. 2005 Sep 2;280(35):30984-93
pubmed: 15987685
J Med Chem. 2017 Dec 28;60(24):10205-10219
pubmed: 29155585
J Med Chem. 2019 Feb 28;62(4):1793-1802
pubmed: 30688459
EMBO J. 1997 May 1;16(9):2352-64
pubmed: 9171349
Mol Cell. 2006 Jun 23;22(6):851-868
pubmed: 16793553
Eur J Med Chem. 2020 Oct 15;204:112657
pubmed: 32738411
Ann N Y Acad Sci. 2010 Dec;1214:99-121
pubmed: 20958325
Cell Rep. 2017 Sep 19;20(12):2876-2890
pubmed: 28930683
J Med Chem. 2019 Feb 14;62(3):1125-1137
pubmed: 30457860
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
J Chem Phys. 2007 Jan 7;126(1):014101
pubmed: 17212484
Blood. 2005 Sep 15;106(6):2183-5
pubmed: 15928039
Nat Immunol. 2013 Dec;14(12):1212-8
pubmed: 24240160
Expert Rev Mol Med. 2004 Jan 30;6(3):1-18
pubmed: 14987415
Nat Med. 2004 Aug;10(8):849-57
pubmed: 15273746
Protein Sci. 1995 Nov;4(11):2411-23
pubmed: 8563639
Cell Rep. 2013 Apr 25;3(4):1293-305
pubmed: 23545499

Auteurs

Sara Bobone (S)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Luca Pannone (L)

Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome 00146, Italy.
Dipartimento di Oncologia e Medicina Molecolare, Istituto Superiore di Sanità, Rome 00161, Italy.

Barbara Biondi (B)

Institute of Biomolecular Chemistry, Padova Unit, CNR, Padova 35131, Italy.

Maja Solman (M)

Hubrecht institute-KNAW and University Medical Center Utrecht, Utrecht 3584 CT, The Netherlands.

Elisabetta Flex (E)

Dipartimento di Oncologia e Medicina Molecolare, Istituto Superiore di Sanità, Rome 00161, Italy.

Viviana Claudia Canale (VC)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Paolo Calligari (P)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Chiara De Faveri (C)

Department of Chemical Sciences, University of Padova, Padova 35131, Italy.

Tommaso Gandini (T)

Department of Chemical Sciences, University of Padova, Padova 35131, Italy.

Andrea Quercioli (A)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Giuseppe Torini (G)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Martina Venditti (M)

Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome 00146, Italy.

Antonella Lauri (A)

Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome 00146, Italy.

Giulia Fasano (G)

Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome 00146, Italy.

Jelmer Hoeksma (J)

Hubrecht institute-KNAW and University Medical Center Utrecht, Utrecht 3584 CT, The Netherlands.

Valerio Santucci (V)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Giada Cattani (G)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Alessio Bocedi (A)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Giovanna Carpentieri (G)

Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome 00146, Italy.
Dipartimento di Oncologia e Medicina Molecolare, Istituto Superiore di Sanità, Rome 00161, Italy.

Valentina Tirelli (V)

Centre of Core Facilities, Istituto Superiore di Sanità, Rome 00161, Italy.

Massimo Sanchez (M)

Centre of Core Facilities, Istituto Superiore di Sanità, Rome 00161, Italy.

Cristina Peggion (C)

Department of Chemical Sciences, University of Padova, Padova 35131, Italy.

Fernando Formaggio (F)

Institute of Biomolecular Chemistry, Padova Unit, CNR, Padova 35131, Italy.
Department of Chemical Sciences, University of Padova, Padova 35131, Italy.

Jeroen den Hertog (J)

Institute of Biomolecular Chemistry, Padova Unit, CNR, Padova 35131, Italy.
Institute of Biology Leiden, Leiden University, Leiden 2333 BE, The Netherlands.

Simone Martinelli (S)

Dipartimento di Oncologia e Medicina Molecolare, Istituto Superiore di Sanità, Rome 00161, Italy.

Gianfranco Bocchinfuso (G)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Marco Tartaglia (M)

Genetics and Rare Diseases Research Division, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome 00146, Italy.

Lorenzo Stella (L)

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Rome 00133, Italy.

Articles similaires

Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH