Phase separation in the outer membrane of
Bacterial Outer Membrane
/ metabolism
Bacterial Outer Membrane Proteins
/ metabolism
Biomolecular Condensates
/ physiology
Cell Membrane
/ metabolism
Cell Wall
/ metabolism
Escherichia coli
/ metabolism
Escherichia coli Proteins
/ metabolism
Lipid Bilayers
/ metabolism
Lipopolysaccharides
/ metabolism
Molecular Dynamics Simulation
Phospholipids
/ metabolism
Porins
/ metabolism
atomic force microscopy
gram-negative bacteria
outer membrane
phase separation
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
02 11 2021
02 11 2021
Historique:
received:
02
07
2021
accepted:
20
09
2021
entrez:
30
10
2021
pubmed:
31
10
2021
medline:
22
12
2021
Statut:
ppublish
Résumé
Gram-negative bacteria are surrounded by a protective outer membrane (OM) with phospholipids in its inner leaflet and lipopolysaccharides (LPS) in its outer leaflet. The OM is also populated with many β-barrel outer-membrane proteins (OMPs), some of which have been shown to cluster into supramolecular assemblies. However, it remains unknown how abundant OMPs are organized across the entire bacterial surface and how this relates to the lipids in the membrane. Here, we reveal how the OM is organized from molecular to cellular length scales, using atomic force microscopy to visualize the OM of live bacteria, including engineered
Identifiants
pubmed: 34716276
pii: 2112237118
doi: 10.1073/pnas.2112237118
pmc: PMC8612244
pii:
doi:
Substances chimiques
Bacterial Outer Membrane Proteins
0
Escherichia coli Proteins
0
Lipid Bilayers
0
Lipopolysaccharides
0
Phospholipids
0
Porins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : F32 GM139232
Pays : United States
Organisme : Medical Research Council
ID : MR/R000328/1
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R35 GM118024
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R000042/1
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors declare no competing interest.
Références
Phys Rev Lett. 2020 Jan 31;124(4):048102
pubmed: 32058787
J Bacteriol. 1981 Jul;147(1):255-8
pubmed: 6263866
Cold Spring Harb Perspect Biol. 2010 May;2(5):a000414
pubmed: 20452953
J Bacteriol. 1977 Jun;130(3):1364-81
pubmed: 405376
Curr Opin Struct Biol. 2015 Dec;35:109-15
pubmed: 26629934
Micron. 2015 May;72:8-14
pubmed: 25725215
Soft Matter. 2016 Mar 14;12(10):2729-36
pubmed: 26931403
FEBS J. 2012 Mar;279(6):894-909
pubmed: 22251410
PLoS One. 2009 Aug 25;4(8):e6739
pubmed: 19707582
J Phys Chem B. 2019 May 2;123(17):3567-3575
pubmed: 30971088
J Biol Chem. 1974 Dec 25;249(24):8019-29
pubmed: 4609976
Mol Microbiol. 2000 Jul;37(2):239-53
pubmed: 10931321
J Bacteriol. 1974 May;118(2):693-707
pubmed: 4133353
Nature. 2018 Jul;559(7715):617-621
pubmed: 30022160
Infect Immun. 2007 Apr;75(4):1661-6
pubmed: 17158899
Biophys J. 2016 Oct 18;111(8):1692-1702
pubmed: 27760356
J Mol Biol. 1989 Sep 5;209(1):171-5
pubmed: 2553985
Biochim Biophys Acta. 1977 Feb 14;465(1):152-64
pubmed: 189812
Analyst. 2019 Nov 18;144(23):6944-6952
pubmed: 31620716
Mol Syst Biol. 2006;2:2006.0008
pubmed: 16738554
Science. 1998 Dec 18;282(5397):2215-20
pubmed: 9856937
mBio. 2020 Feb 25;11(1):
pubmed: 32098817
Methods. 2021 Sep;193:68-79
pubmed: 33548405
J Bacteriol. 2005 Mar;187(6):1913-22
pubmed: 15743937
Biochim Biophys Acta. 1983 Mar 21;737(1):51-115
pubmed: 6337630
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Mol Microbiol. 2009 Dec;74(5):1211-22
pubmed: 19843216
Proc Natl Acad Sci U S A. 2009 May 12;106(19):8009-14
pubmed: 19383799
mBio. 2020 Apr 14;11(2):
pubmed: 32291302
J Biol Chem. 2020 Jul 3;295(27):9147-9156
pubmed: 32398259
Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5034-43
pubmed: 27493217
J Mol Biol. 2012 Sep 14;422(2):300-9
pubmed: 22613761
Antimicrob Agents Chemother. 1993 Nov;37(11):2255-60
pubmed: 8285603
Cell Rep. 2018 May 29;23(9):2782-2794
pubmed: 29847806
Front Cell Infect Microbiol. 2017 May 29;7:220
pubmed: 28611954
Gene. 1995 May 26;158(1):9-14
pubmed: 7789817
J Bacteriol. 1978 Jun;134(3):1089-98
pubmed: 350838
Science. 1995 Apr 7;268(5207):92-4
pubmed: 7701347
PLoS Comput Biol. 2012;8(9):e1002680
pubmed: 23028278
EMBO J. 2019 Feb 15;38(4):
pubmed: 30643019
Nat Commun. 2018 Mar 14;9(1):1082
pubmed: 29540681
J Bacteriol. 1996 Jul;178(14):4182-8
pubmed: 8763947
Biophys J. 1994 Dec;67(6):2394-403
pubmed: 7696479
J Biol Chem. 2020 Jul 24;295(30):10340-10367
pubmed: 32499369
Nat Commun. 2018 Jul 20;9(1):2846
pubmed: 30030429
J Bacteriol. 1970 Jan;101(1):304-13
pubmed: 4189229
Nature. 2015 Jul 16;523(7560):333-6
pubmed: 26061769
Structure. 1995 Oct 15;3(10):1041-50
pubmed: 8589999
Nature. 1980 May 22;285(5762):261-3
pubmed: 6990276
Biochemistry. 2006 Aug 15;45(32):9639-47
pubmed: 16893165
Nat Commun. 2020 Mar 11;11(1):1305
pubmed: 32161270
Proc Natl Acad Sci U S A. 2018 Aug 7;115(32):E7587-E7594
pubmed: 30037998
Mol Microbiol. 2015 Dec;98(6):1133-46
pubmed: 26314242
J Mol Biol. 1983 Apr 25;165(4):701-10
pubmed: 6304320