Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins.
Adhesion
Anopheles
Malaria
Migration
Transmission
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
30 Oct 2021
30 Oct 2021
Historique:
received:
27
05
2021
accepted:
19
10
2021
entrez:
31
10
2021
pubmed:
1
11
2021
medline:
30
11
2021
Statut:
epublish
Résumé
Plasmodium sporozoites are the highly motile forms of malaria-causing parasites that are transmitted by the mosquito to the vertebrate host. Sporozoites need to enter and cross several cellular and tissue barriers for which they employ a set of surface proteins. Three of these proteins are members of the thrombospondin related anonymous protein (TRAP) family. Here, potential additive, synergistic or antagonistic roles of these adhesion proteins were investigated. Four transgenic Plasmodium berghei parasite lines that lacked two or all three of the TRAP family adhesins TRAP, TLP and TREP were generated using positive-negative selection. The parasite lines were investigated for their capacity to attach to and move on glass, their ability to egress from oocysts and their capacity to enter mosquito salivary glands. One strain was in addition interrogated for its capacity to infect mice. The major phenotype of the TRAP single gene deletion dominates additional gene deletion phenotypes. All parasite lines including the one lacking all three proteins were able to conduct some form of active, if unproductive movement. The individual TRAP-family adhesins appear to play functionally distinct roles during motility and infection. Other proteins must contribute to substrate adhesion and gliding motility.
Sections du résumé
BACKGROUND
BACKGROUND
Plasmodium sporozoites are the highly motile forms of malaria-causing parasites that are transmitted by the mosquito to the vertebrate host. Sporozoites need to enter and cross several cellular and tissue barriers for which they employ a set of surface proteins. Three of these proteins are members of the thrombospondin related anonymous protein (TRAP) family. Here, potential additive, synergistic or antagonistic roles of these adhesion proteins were investigated.
METHODS
METHODS
Four transgenic Plasmodium berghei parasite lines that lacked two or all three of the TRAP family adhesins TRAP, TLP and TREP were generated using positive-negative selection. The parasite lines were investigated for their capacity to attach to and move on glass, their ability to egress from oocysts and their capacity to enter mosquito salivary glands. One strain was in addition interrogated for its capacity to infect mice.
RESULTS
RESULTS
The major phenotype of the TRAP single gene deletion dominates additional gene deletion phenotypes. All parasite lines including the one lacking all three proteins were able to conduct some form of active, if unproductive movement.
CONCLUSIONS
CONCLUSIONS
The individual TRAP-family adhesins appear to play functionally distinct roles during motility and infection. Other proteins must contribute to substrate adhesion and gliding motility.
Identifiants
pubmed: 34717635
doi: 10.1186/s12936-021-03960-3
pii: 10.1186/s12936-021-03960-3
pmc: PMC8557484
doi:
Substances chimiques
Protozoan Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
430Subventions
Organisme : Human Frontier Science Program
ID : RGY0071/2011
Organisme : European Research Council
ID : StG 281719
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : 240245660
Informations de copyright
© 2021. The Author(s).
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