Hybridoma Screening by Antibody Capture: Dot Blot.

Antibodies, Monoclonal Antigens Clone Cells Collodion Hybridomas Immunoblotting

Journal

Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530

Informations de publication

Date de publication:
01 11 2021
Historique:
entrez: 2 11 2021
pubmed: 3 11 2021
medline: 23 4 2022
Statut: epublish

Résumé

A dot blot is an appropriate hybridoma screening procedure when the antigen is a protein that is available in purified form. The antigen is bound directly to a nitrocellulose sheet and incubated with hybridoma tissue culture supernatant. A dot blot is widely used to determine the productivity of a given hybridoma, and this is described here. This assay can also be used to screen a fusion or subclone plate for productive hybridoma clones.

Identifiants

DOI: 10.1101/pdb.prot103051 PMID: 34725173
pubmed: 34725173
pii: 2021/11/pdb.prot103051
doi: 10.1101/pdb.prot103051
doi:

Substances chimiques

Antibodies, Monoclonal 0
Antigens 0
Collodion 9004-70-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2021 Cold Spring Harbor Laboratory Press.

Auteurs

Edward A Greenfield (EA)

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Hybridoma Screening by Antibody Capture: Dot Blot.
questionsmedicales.fr › Facteurs biologiques › Antigènes › Hybridoma Screening by Antibody Capture: Dot Blot.
questionsmedicales.fr › Cellules › Cellules cultivées › Clones cellulaires › Hybridoma Screening by Antibody Capture: Dot Blot.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Biopolymères › Cellulose › Collodion › Hybridoma Screening by Antibody Capture: Dot Blot.
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules hybrides › Hybridomes › Hybridoma Screening by Antibody Capture: Dot Blot.
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Hybridoma Screening by Antibody Capture: Dot Blot.