Soy diet induces intestinal inflammation in adult Zebrafish: Role of OTX and P53 family.
Animals
Anti-Inflammatory Agents
/ pharmacology
Colon
/ metabolism
Diet
Disease Models, Animal
Inflammation
/ metabolism
Inflammatory Bowel Diseases
/ immunology
Intestinal Mucosa
/ immunology
Intestines
/ metabolism
Glycine max
Tumor Necrosis Factor-alpha
/ metabolism
Tumor Suppressor Protein p53
/ metabolism
Zebrafish
gut
inflammation
otx
p53
p63
p73
soy
zebrafish
Journal
International journal of experimental pathology
ISSN: 1365-2613
Titre abrégé: Int J Exp Pathol
Pays: England
ID NLM: 9014042
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
revised:
10
09
2021
received:
29
03
2021
accepted:
07
10
2021
pubmed:
3
11
2021
medline:
10
2
2022
entrez:
2
11
2021
Statut:
ppublish
Résumé
Inflammatory bowel diseases (IBDs) are a group of inflammatory conditions of the colon and small intestine, including Crohn's disease and ulcerative colitis. Since Danio rerio is a promising animal model to study gut function, we developed a soy-dependent model of intestinal inflammation in adult zebrafish. The soya bean meal diet was given for 4 weeks and induced an inflammatory process, as demonstrated by morphological changes together with an increased percentage of neutrophils infiltrating the intestinal wall, which developed between the second and fourth week of treatment. Pro-inflammatory genes such as interleukin-1beta, interleukin-8 and tumour necrosis factor alpha were upregulated in the second week and anti-inflammatory genes such as transforming growth factor beta and interleukin-10. Interestingly, an additional expression peak was found for interleukin-8 at the fourth week. Neuronal genes, OTX1 and OTX2, were significantly upregulated in the first two weeks, compatible with the development of the changes in the gut wall. As for the genes of the p53 family such as p53, DNp63 and p73, a statistically significant increase was observed after two weeks of treatment compared with controls. Interestingly, DNp63 and p73 were shown an additional peak after four weeks. Our data demonstrate that soya bean meal diet negatively influences intestinal morphology and immunological function in adult zebrafish showing the features of acute inflammation. Data observed at the fourth week of treatment may suggest initiation of chronic inflammation. Adult zebrafish may represent a promising model to better understand the mechanisms of food-dependent intestinal inflammation.
Identifiants
pubmed: 34725870
doi: 10.1111/iep.12420
pmc: PMC8781668
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Tumor Necrosis Factor-alpha
0
Tumor Suppressor Protein p53
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13-22Informations de copyright
© 2021 The Authors. International Journal of Experimental Pathology published by John Wiley & Sons Ltd on behalf of Company of the International Journal of Experimental Pathology (CIJEP).
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