Proteogenomic discovery of sORF-encoded peptides associated with bacterial virulence in Yersinia pestis.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
02 11 2021
02 11 2021
Historique:
received:
16
01
2021
accepted:
08
10
2021
entrez:
3
11
2021
pubmed:
4
11
2021
medline:
21
12
2021
Statut:
epublish
Résumé
Plague caused by Yersinia pestis is one of the deadliest diseases. However, many molecular mechanisms of bacterial virulence remain unclear. This study engaged in the discovery of small open reading frame (sORF)-encoded peptides (SEPs) in Y. pestis. An integrated proteogenomic pipeline was established, and an atlas containing 76 SEPs was described. Bioinformatic analysis indicated that 20% of these SEPs were secreted or localized to the transmembrane and that 33% contained functional domains. Two SEPs, named SEPs-yp1 and -yp2 and encoded in noncoding regions, were selected by comparative peptidomics analysis under host-specific environments and high-salinity stress. They displayed important roles in the regulation of antiphagocytic capability in a thorough functional assay. Remarkable attenuation of virulence in mice was observed in the SEP-deleted mutants. Further global proteomic analysis indicated that SEPs-yp1 and -yp2 affected the bacterial metabolic pathways, and SEP-yp1 was associated with the bacterial virulence by modulating the expression of key virulence factors of the Yersinia type III secretion system. Our study provides a rich resource for research on Y. pestis and plague, and the findings on SEP-yp1 and SEP-yp2 shed light on the molecular mechanism of bacterial virulence.
Identifiants
pubmed: 34728737
doi: 10.1038/s42003-021-02759-x
pii: 10.1038/s42003-021-02759-x
pmc: PMC8563848
doi:
Substances chimiques
Bacterial Proteins
0
Peptides
0
Virulence Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1248Subventions
Organisme : Ministry of Science and Technology of the People's Republic of China (Chinese Ministry of Science and Technology)
ID : 2016YFA0501302
Informations de copyright
© 2021. The Author(s).
Références
Nat Protoc. 2016 May;11(5):993-1006
pubmed: 27123950
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
Nucleic Acids Res. 2019 Sep 5;47(15):8111-8125
pubmed: 31340039
Infect Immun. 2018 May 22;86(6):
pubmed: 29610260
Infect Immun. 1986 Feb;51(2):445-54
pubmed: 3002984
Curr Opin Microbiol. 2017 Oct;39:81-88
pubmed: 29111488
J Bacteriol. 2017 May 9;199(11):
pubmed: 28289085
Trends Cell Biol. 2017 Sep;27(9):685-696
pubmed: 28528987
Genome Biol. 2009;10(3):R25
pubmed: 19261174
Nat Biotechnol. 2010 May;28(5):511-5
pubmed: 20436464
Trends Microbiol. 2016 Apr;24(4):239-241
pubmed: 26875618
Mol Syst Biol. 2019 Feb 18;15(2):e8503
pubmed: 30777892
Anal Chem. 2017 Dec 5;89(23):12909-12917
pubmed: 29090900
J Proteome Res. 2014 Mar 7;13(3):1757-65
pubmed: 24490786
Annu Rev Biochem. 2014;83:753-77
pubmed: 24606146
Microbes Infect. 2007 Feb;9(2):224-33
pubmed: 17223369
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Immunology. 2009 Sep;128(1 Suppl):e406-17
pubmed: 19191914
J Bacteriol. 2020 May 11;202(11):
pubmed: 32205462
Trends Genet. 2000 Jun;16(6):276-7
pubmed: 10827456
Front Cell Infect Microbiol. 2013 Dec 24;3:106
pubmed: 24400226
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W29-37
pubmed: 21593126
J Mol Biol. 2004 May 14;338(5):1027-36
pubmed: 15111065
J Bacteriol. 2010 Mar;192(6):1685-99
pubmed: 20061468
Nat Commun. 2020 Jan 15;11(1):281
pubmed: 31941912
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
pubmed: 10829079
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Nat Biotechnol. 2019 Apr;37(4):420-423
pubmed: 30778233
Cell Adh Migr. 2014;8(4):404-17
pubmed: 25482634
J Bacteriol. 2002 Aug;184(16):4601-11
pubmed: 12142430
PLoS One. 2010 Sep 17;5(9):
pubmed: 20862262
Nat Chem Biol. 2013 Jan;9(1):59-64
pubmed: 23160002
Cell. 2019 Aug 22;178(5):1245-1259.e14
pubmed: 31402174
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613
pubmed: 30476243
J Biol Chem. 2017 Mar 31;292(13):5488-5498
pubmed: 28196868
DNA Res. 2004 Jun 30;11(3):179-97
pubmed: 15368893
Nature. 2001 Oct 4;413(6855):523-7
pubmed: 11586360
J Mol Biol. 2001 Jan 19;305(3):567-80
pubmed: 11152613
Nucleic Acids Res. 2016 Jan 4;44(D1):D279-85
pubmed: 26673716
Front Microbiol. 2015 Feb 12;6:110
pubmed: 25729381
Front Cell Infect Microbiol. 2017 Dec 11;7:505
pubmed: 29312891
J Bacteriol. 2004 Aug;186(15):5147-52
pubmed: 15262951
Mol Microbiol. 2001 Apr;40(1):20-36
pubmed: 11298273
BMC Bioinformatics. 2010 Mar 08;11:119
pubmed: 20211023
Mol Microbiol. 2013 Jan;87(2):301-17
pubmed: 23205707
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
Mol Syst Biol. 2019 Feb 22;15(2):e8290
pubmed: 30796087
Nucleic Acids Res. 2018 Jan 4;46(D1):D497-D502
pubmed: 29140531
Res Microbiol. 2005 Apr;156(3):403-15
pubmed: 15808945
J Bacteriol. 2009 Aug;191(15):4714-21
pubmed: 19465648
PLoS Pathog. 2016 Mar 29;12(3):e1005530
pubmed: 27022930
J Clin Microbiol. 2017 Dec 26;56(1):
pubmed: 29070654
Front Cell Infect Microbiol. 2013 Feb 06;3:4
pubmed: 23390616