The use of fly artifacts in a crime scene: Is there any application for forensic toxicology?
6-MAM
benzoylecgonine
codeine
crime scene investigation
fly artifacts
forensic toxicology
human bloodstains
liquid chromatography-mass spectrometry
Journal
Journal of forensic sciences
ISSN: 1556-4029
Titre abrégé: J Forensic Sci
Pays: United States
ID NLM: 0375370
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
18
10
2021
received:
06
09
2021
accepted:
18
10
2021
pubmed:
4
11
2021
medline:
7
4
2022
entrez:
3
11
2021
Statut:
ppublish
Résumé
Fly artifacts (FA) are bloodstains resulting from insect activity at a crime scene, usually by feeding on human blood. Whether these artifactual stains might be useful for forensic toxicological investigations in cases of absence of conventional and unconventional matrices, for example, in cases concealment of the body or of extensive putrefaction, has not yet been investigated. The purpose of this study is to understand if FA trace evidence permits toxicological analysis when traditional matrices are not available. To this aim, FA experimentally produced by Calliphora vomitoria feeding on human blood of a cocaine and heroin user were collected from absorptive and non-absorptive material. FA material was analyzed by a new simple and fast LC-MS/MS method. Results were evaluated in terms of presence of the drug and relative amount of the detected molecules. From a qualitative point of view, the analysis of FA revealed all the substances originally detected in post-mortem blood in both cases. The ratios of cocaine/benzoylecgonine, codeine/morphine, and 6-monoacetylmorphine/morphine recovered in FA from cotton-textile materials and from non-absorptive surfaces were consistent with data resulted from original post-mortem blood. The preliminary study herein reported demonstrated that FA are extremely informative in case of cocaine and heroin users and merit further research in order to be applied in real caseworks.
Identifiants
pubmed: 34729777
doi: 10.1111/1556-4029.14930
doi:
Substances chimiques
Morphine Derivatives
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
749-755Informations de copyright
© 2021 American Academy of Forensic Sciences.
Références
Peschel O, Kunz SN, Rothschild MA, Mützel E. Blood stain pattern analysis. Forensic Sci Med Pathol. 2011;7(3):257-70. https://doi.org/10.1007/s12024-010-9198-1
Bevel T, Gardner R. Bloodstain pattern analysis with an introduction to crime scene reconstruction. 3rd edn. Boca Raton, FL: CRC Press; 2008. p. 149-63.
Dissing J, Søndervang A, Lund S. Exploring the limits for the survival of DNA in blood stains. J Forensic Leg Med. 2010;17(7):392-6. https://doi.org/10.1016/j.jflm.2010.08.001
Kim J-Y, Park J-H, Kim MI, Lee HH, Kim HL, Jeong K-S, et al. Identification of female-specific blood stains using a 17β-estradiol-targeted aptamer-based sensor. Int J Legal Med. 2018;132(1):91-8. https://doi.org/10.1007/s00414-017-1718-z
Chace DH, Lappas NT. The use of dried blood spots and stains in forensic science. In: Li W, Lee MS, editors. Dried blood spots: Applications and techniques. Hoboken, NJ: Wiley; 2014. p. 140-50. https://doi.org/10.1002/9781118890837.ch11
Krueger J, Sachs H, Graw M, Musshoff F, Roider G. Toxicological analysis of bloodstains and traces. Toxichem Krimtech. 2017;84(3):171-5.
Rivers DB, Dahlem GA. The science of forensic entomology. Hoboken, NJ: Wiley-Blackwell; 2014. p. 237-46.
Viero A, Montisci M, Pelletti G, Vanin S. Crime scene and body alterations caused by arthropods: implications in death investigation. Int J Legal Med. 2019;133(1):307-16. https://doi.org/10.1007/s00414-018-1883-8
Rivers D, Geiman T. Insect artifacts are more than just altered bloodstains. Insects. 2017;8(2):37. https://doi.org/10.3390/insects8020037
Rivers DB, Dunphy B, Hammerschmidt C, Carrigan A. Characterization of insect stains deposited by Calliphora vicina (Diptera: Calliphoridae) on shirt fabrics. J Med Entomol. 2020;57(5): 1399-406. https://doi.org/10.1093/jme/tjaa052
Fujikawa A, Barksdale L, Higley LG, Carter DO. Changes in the morphology and presumptive chemistry of impact and pooled bloodstain patterns by Lucilia sericata (Meigen) (Diptera: Calliphoridae). J Forensic Sci. 2011;56(5):1315-8. https://doi.org/10.1111/j.1556-4029.2011.01800
Ristenbatt RR. Commentary on: Rivers DB, et al. Immunoassay detection of fly artifacts produced by several species of necrophagous flies following feeding on human blood. Forensic Sci Int Synerg. 2019;1(1):1-10. Forensic Sci Int Synerg. 2019;1:303-4. https://doi.org/10.1016/j.fsisyn.2019.04.007
Pelletti G, Mazzotti MC, Fais P, Martini D, Ingrà L, Amadasi A, et al. Scanning electron microscopy in the identification of fly artifacts. Int J Legal Med. 2019;133(5):1575-80. https://doi.org/10.1007/s00414-019-02090-5
Bini C, Giorgetti A, Iuvaro A, Giovannini E, Gianfreda D, Pelletti G, et al. A DNA-based method for distinction of fly artifacts from human bloodstains. Int J Legal Med. 2021;135(6):2155-61. https://doi.org/10.1007/s00414-021-02643-7
Pelletti G, Martini D, Ingrà L, Mazzotti MC, Giorgetti A, Falconi M, et al. Morphological characterization using scanning electron microscopy of fly artifacts deposited by Calliphora vomitoria (Diptera: Calliphoridae) on household materials. Int J Legal Med. https://doi.org/10.1007/s00414-021-02634-8. Epub 2021 Jul 1.
Benecke M, Barksdale L. Distinction of bloodstain patterns from fly artifacts. Forensic Sci Int. 2003;137(2-3):152-9. https://doi.org/10.1016/j.forsciint.2003.07.012
Alfazil AA, Anderson RA. Stability of benzodiazepines and cocaine in blood spots stored on filter paper. J Anal Toxicol. 2008;32(7):511-5. https://doi.org/10.1093/jat/32.7.511
Moretti M, Visonà SD, Freni F, Tomaciello I, Vignali C, Groppi A, et al. A liquid chromatography-tandem mass spectrometry method for the determination of cocaine and metabolites in blood and in dried blood spots collected from postmortem samples and evaluation of the stability over a 3-month period. Drug Test Anal. 2018;10:1430-7. https://doi.org/10.1002/dta.2399
Boy RG, Henseler J, Mattern R, Skopp G. Determination of morphine and 6-acetylmorphine in blood with use of dried blood spots. Ther Drug Monit. 2008;30(6):733-9. https://doi.org/10.1097/ftd.0b013e31818d9fdb
Gladstone M, Su TT. Chemical genetics and drug screening in Drosophila cancer models. J Genet Genomics. 2011;38(10):497-504. https://doi.org/10.1016/j.jgg.2011.09.003
Idda T, Bonas C, Hoffmann J, Bertram J, Quinete N, Schettgen T, et al. Metabolic activation and toxicological evaluation of polychlorinated biphenyls in Drosophila melanogaster. Sci Rep. 2020;10(1):21587. https://doi.org/10.1038/s41598-020-78405-z