Virus self-assembly proceeds through contact-rich energy minima.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
05 Nov 2021
05 Nov 2021
Historique:
entrez:
3
11
2021
pubmed:
4
11
2021
medline:
4
11
2021
Statut:
ppublish
Résumé
Self-assembly of supramolecular complexes such as viral capsids occurs prominently in nature. Nonetheless, the mechanisms underlying these processes remain poorly understood. Here, we uncover the assembly pathway of hepatitis B virus (HBV), applying fluorescence optical tweezers and high-speed atomic force microscopy. This allows tracking the assembly process in real time with single-molecule resolution. Our results identify a specific, contact-rich pentameric arrangement of HBV capsid proteins as a key on-path assembly intermediate and reveal the energy balance of the self-assembly process. Real-time nucleic acid packaging experiments show that a free energy change of ~1.4
Identifiants
pubmed: 34730996
doi: 10.1126/sciadv.abg0811
pmc: PMC8565845
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
eabg0811Subventions
Organisme : NIAID NIH HHS
ID : R01 AI144022
Pays : United States
Références
J Mol Biol. 1974 Jun 25;86(2):469-89
pubmed: 4416620
Curr Opin Struct Biol. 2000 Jun;10(3):279-85
pubmed: 10851197
J Mol Biol. 2018 Aug 3;430(16):2453-2467
pubmed: 29045818
Biophys J. 2015 Jan 6;108(1):14-6
pubmed: 25564845
Sci Adv. 2020 Apr 15;6(16):eaaz1639
pubmed: 32494611
Phys Rev Lett. 2016 Jun 24;116(25):258102
pubmed: 27391755
J Am Chem Soc. 2019 Jan 23;141(3):1251-1260
pubmed: 30537810
Biophys J. 2006 Mar 15;90(6):1939-48
pubmed: 16387781
Methods Enzymol. 2017;582:85-119
pubmed: 28062046
Nat Chem. 2011 Feb;3(2):126-32
pubmed: 21258385
Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):11329-34
pubmed: 25034253
J Mol Biol. 2014 Sep 9;426(18):3148-3165
pubmed: 25036288
Biophys J. 2010 Mar 17;98(6):1065-74
pubmed: 20303864
J Gen Virol. 1979 Nov;45(2):509-14
pubmed: 541667
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
PLoS Pathog. 2012 Sep;8(9):e1002919
pubmed: 23028319
ACS Nano. 2020 Jul 28;14(7):8724-8734
pubmed: 32633498
Science. 1996 Feb 9;271(5250):795-9
pubmed: 8628994
Biochemistry. 2002 Oct 1;41(39):11525-31
pubmed: 12269796
Nat Commun. 2015 Jun 18;6:7304
pubmed: 26084388
Nat Commun. 2012;3:1013
pubmed: 22910359
J Virol. 2010 Jul;84(14):7174-84
pubmed: 20427522
Biopolymers. 2013 Sep;99(9):611-20
pubmed: 23444293
Biopolymers. 2010 Jan;93(1):1-7
pubmed: 19722180
Nat Commun. 2018 Aug 6;9(1):3071
pubmed: 30082710
Nature. 2009 Jan 22;457(7228):446-50
pubmed: 19129763
J Am Chem Soc. 2020 Aug 12;142(32):13709-13717
pubmed: 32786814
Cell. 1994 Jun 17;77(6):943-50
pubmed: 8004680
J Am Chem Soc. 2020 Apr 29;142(17):7868-7882
pubmed: 32233479
J Virol. 1992 Jul;66(7):4107-16
pubmed: 1602535
Annu Rev Phys Chem. 2015 Apr;66:217-39
pubmed: 25532951
Integr Biol (Camb). 2015 Sep;7(9):967-86
pubmed: 26287962
Biochemistry. 1999 Nov 2;38(44):14644-52
pubmed: 10545189
Nucleic Acids Res. 2013 Jan 7;41(1):167-81
pubmed: 23143110
Virology. 2012 Aug 15;430(1):20-9
pubmed: 22595445
PLoS Comput Biol. 2020 Apr 20;16(4):e1007782
pubmed: 32310951
Nature. 2007 Dec 13;450(7172):973-82
pubmed: 18075576
Virus Res. 2008 Jun;134(1-2):235-49
pubmed: 18339439
J Virol. 2014 Mar;88(5):2530-43
pubmed: 24352445
Nucleic Acids Res. 2020 Apr 6;48(6):e34
pubmed: 32016413
Annu Rev Biochem. 2004;73:705-48
pubmed: 15189157
Nucleic Acids Res. 2014 Feb;42(3):2064-74
pubmed: 24225314
J Virol. 2016 May 27;90(12):5830-5844
pubmed: 27076641
Nano Lett. 2019 Aug 14;19(8):5746-5753
pubmed: 31368710
Elife. 2018 Jan 29;7:
pubmed: 29377794
J Biomol Struct Dyn. 2020 Sep;38(14):4289-4303
pubmed: 31570067
Sci Rep. 2017 Feb 13;7:42374
pubmed: 28205569
Biophys J. 2010 Sep 8;99(5):1350-7
pubmed: 20816046
J Am Chem Soc. 2018 May 2;140(17):5784-5790
pubmed: 29672035
Biochemistry. 2009 Mar 3;48(8):1736-42
pubmed: 19196007
Sci Adv. 2019 Apr 10;5(4):eaau7198
pubmed: 30989108
Nat Protoc. 2012 May 24;7(6):1193-206
pubmed: 22635111
Structure. 1995 Oct 15;3(10):1009-19
pubmed: 8589996
Biochemistry. 1995 Apr 18;34(15):4919-32
pubmed: 7711014
Biophys Rev. 2016 Nov 15;9(1):17-40
pubmed: 28303166
ACS Nano. 2019 Jul 23;13(7):7610-7626
pubmed: 31173689
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2018 Jan;10(1):
pubmed: 28719102
J Virol. 2006 Nov;80(22):11055-61
pubmed: 16943288
Nucleic Acids Res. 2018 Apr 20;46(7):3633-3642
pubmed: 29897602
Nat Rev Phys. 2021;3(2):76-91
pubmed: 33728406
Proc Natl Acad Sci U S A. 2019 Nov 5;116(45):22485-22490
pubmed: 31570619
ACS Nano. 2013 Oct 22;7(10):8447-54
pubmed: 24010404
J Mol Biol. 1994 Aug 5;241(1):59-67
pubmed: 8051707
Nano Lett. 2007 Feb;7(2):338-44
pubmed: 17297998
Biophys J. 2002 Aug;83(2):1217-30
pubmed: 12124301
Nature. 2017 Dec 21;552(7685):415-420
pubmed: 29236688
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2020 Jul;12(4):e1613
pubmed: 31960585
J Virol. 2010 Feb;84(3):1607-15
pubmed: 19939922
J Am Chem Soc. 2018 Nov 14;140(45):15261-15269
pubmed: 30375863
Nature. 2006 Aug 10;442(7103):709-12
pubmed: 16799566