Metabolomics reveals the mechanism of Antarctic yeast Rhodotorula mucliaginosa AN5 to cope with cadmium stress.
Adaptive resistance
GC–MS
Heavy metal pollution
Metabolomics
Rhodotorula mucilaginosa
Journal
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine
ISSN: 1572-8773
Titre abrégé: Biometals
Pays: Netherlands
ID NLM: 9208478
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
received:
01
04
2021
accepted:
25
10
2021
pubmed:
4
11
2021
medline:
6
5
2022
entrez:
3
11
2021
Statut:
ppublish
Résumé
Heavy metal pollution in Antarctica has far exceeded expectations. Antarctic yeast is widely present in polar marine environment. The mechanisms of metabolomics effect of heavy metal on polar yeast have not been reported previously. In this study, gas chromatography-mass spectrometry (GC-MS) wascarried out to performed the metabolite profiling analysis of Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5 exposed to different cadmium (Cd) stresses of 5 mM (HM5), 10 mM (HM10) and 20 mM (HM20), respectively. Metabolic profile analysis showed that the composition and contents of cellular metabolites have been altered by cadmium. 93 different metabolites were identified altogether, among which 23, 58 and 81 different metabolites were found in HM5, HM10 and HM20 group respectively. MetaboAnalyst analysis showed that in HM5, HM10 and HM20 groups, 12, 24 and 31 metabolic pathways were involved in the stress of cadmium to R. mucilaginosa, respectively. By contrasting with Kyoto Encyclopedia of Genes and Genomes database, we discovered that exposure of yeast AN5 to Cd stress resulted in profound biochemical changes including amino acids, organic acids and saccharides. These results will supply a nonnegligible basis of studying the adaptive resistance mechanism of Antarctic yeast Rhodotorula mucilaginosa to heavy metal.
Identifiants
pubmed: 34731410
doi: 10.1007/s10534-021-00350-9
pii: 10.1007/s10534-021-00350-9
doi:
Substances chimiques
Metals, Heavy
0
Tetrahydroisoquinolines
0
Cadmium
00BH33GNGH
gastrofenzin
89845-17-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
53-65Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
Références
Bankar A, Zinjarde S, Shinde M, Gopalghare G, Ravikumar A (2018) Heavy metal tolerance in marine strains of Yarrowia lipolytica. Extremophiles 22(4):617–628
pubmed: 29594464
Bartel J, Krumsiek J, Theis FJ (2013) Statistical methods for the analysis of high-throughput metabolomics data. Comput Struct Biotechnol J 4:e201301009
pubmed: 24688690
pmcid: 3962125
Bedard K, Krause KH (2007) The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology. Physiol Rev 87(1):245–313
pubmed: 17237347
Bujak R, Struck-Lewicka W, Markuszewski MJ, Kaliszan R (2015) Metabolomics for laboratory diagnostics. J Pharm Biomed Anal 113:108–120
pubmed: 25577715
Chandra K, Salman A, Mohd A, Sweety R, Ali KN (2015) Protection against FCA induced oxidative stress induced DNA damage as a model of arthritis and in vitro anti-arthritic potential of Costus speciosus rhizome extract. Int J Pharmacogn Phytochem Res 7(2):383–389
Cheng J, Qiu H, Chang Z, Jiang Z, Yin W (2016) The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris. Springerplus 5(1):1290
pubmed: 27652004
pmcid: 5017993
Chong J, Yamamoto M (2019) MetaboAnalystR 2.0: from raw spectra to biological insights. 9(3).
Courant F, Antignac JP, Dervilly-Pinel G, Le Bizec B (2014) Basics of mass spectrometry based metabolomics. Proteomics 14(21–22):2369–2388
pubmed: 25168716
Fernandez PM, Martorell MM, Blaser MG, Ruberto LAM, de Figueroa LIC, Mac Cormack WP (2017) Phenol degradation and heavy metal tolerance of Antarctic yeasts. Extremophiles 21(3):445–457
pubmed: 28271165
Fiehn O (2016) Metabolomics by gas chromatography-mass spectrometry: combined targeted and untargeted profiling. Curr Protoc Mol Biol 114:30.34.31-30.34.32
Gong X, Huang D, Liu Y, Zeng G, Wang R, Wan J, Zhang C, Cheng M, Qin X, Xue W (2017) Stabilized nanoscale zerovalent iron mediated cadmium accumulation and oxidative damage of Boehmeria nivea (L.) Gaudich cultivated in cadmium contaminated sediments. Environ Sci Technol 51(19):11308–11316
pubmed: 28850225
Hosono T, Su C-C, Delinom R, Umezawa Y, Toyota T, Kaneko S, Taniguchi M (2011) Decline in heavy metal contamination in marine sediments in Jakarta Bay, Indonesia due to increasing environmental regulations. Estuar Coast Shelf Sci 92(2):297–306
Jamers A, Blust R, De Coen W, Griffin JL, Jones OA (2013) An omics based assessment of cadmium toxicity in the green alga Chlamydomonas reinhardtii. Aquat Toxicol 126:355–364
pubmed: 23063003
Jia G, Sha K, Feng X, Liu H (2019) Post-thawing metabolite profile and amino acid oxidation of thawed pork tenderloin by HVEF-A short communication. Food Chem 291:16–21
pubmed: 31006455
Ju Y (2019) iTRAQ Quantitative proteomics study of Rhodotorula Antarctica AN5 under cadmium stress (Master's Thesis, Harbin Institute of Technology). https://kns.cnki.net/KCMS/detail/detail.aspx?dbname=CMFD202101&filename=1020397370.nh
Kan G, Wang X, Jiang J, Zhang C, Chi M, Ju Y, Shi C (2019) Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic. Microbiologyopen 8(3):e00657
pubmed: 29926536
Kim J (2019) Sugar metabolism as input signals and fuel for leaf senescence. Genes Genomics 41(7):737–746
pubmed: 30879182
Laíns I, Gantner M, Murinello S, Lasky-Su JA, Miller JW, Friedlander M, Husain D (2019) Metabolomics in the study of retinal health and disease. Prog Retin Eye Res 69:57–79
pubmed: 30423446
Lavoie M, Le Faucheur S, Fortin C, Campbell PG (2009) Cadmium detoxification strategies in two phytoplankton species: metal binding by newly synthesized thiolated peptides and metal sequestration in granules. Aquat Toxicol 92(2):65–75
pubmed: 19201040
Li H, Ma ML, Luo S, Zhang RM, Han P, Hu W (2012) Metabolic responses to ethanol in Saccharomyces cerevisiae using a gas chromatography tandem mass spectrometry-based metabolomics approach. Int J Biochem Cell Biol 44(7):1087–1096
pubmed: 22504284
Liu J, Li J, Shin HD, Liu L, Du G, Chen J (2017) Protein and metabolic engineering for the production of organic acids. Bioresour Technol 239:412–421
pubmed: 28538198
Mangal V, Nguyen TQ, Fiering Q, Guéguen C (2020) An untargeted metabolomic approach for the putative characterization of metabolites from Scenedesmus obliquus in response to cadmium stress. Environ Pollut 266(Pt 2):115123
pubmed: 32688078
Mani S (2015) Production of reactive oxygen species and its implication in human diseases. In: Rani V, Yadav UCS (eds) Free radicals in human health and disease. Springer, New Delhi, pp 3–15
Marina-Montes C, Pérez-Arribas LV, Escudero M, Anzano J, Cáceres JO (2020) Heavy metal transport and evolution of atmospheric aerosols in the Antarctic region. Sci Total Environ 721:137702
pubmed: 32169645
Morgan-Kiss RM, Priscu JC, Pocock T, Gudynaite-Savitch L, Huner NP (2006) Adaptation and acclimation of photosynthetic microorganisms to permanently cold environments. Microbiol Mol Biol Rev 70(1):222–252
pubmed: 16524924
pmcid: 1393254
Ojuederie OB, Babalola OO (2017) Microbial and plant-assisted bioremediation of heavy metal polluted environments: a review. IJERPH 14(12):1504
pmcid: 5750922
Oliver SG, Winson MK, Kell DB, Baganz F (1998) Systematic functional analysis of the yeast genome. Trends Biotechnol 16(9):373–378
pubmed: 9744112
Orlando GF, Wolf G, Engelmann M (2008) Role of neuronal nitric oxide synthase in the regulation of the neuroendocrine stress response in rodents: insights from mutant mice. Amino Acids 35(1):17–27
pubmed: 18301960
Park MH, Cooper HL, Folk JE (1981) Identification of hypusine, an unusual amino acid, in a protein from human lymphocytes and of spermidine as its biosynthetic precursor. Proc Natl Acad Sci USA 78(5):2869–2873
pubmed: 6789324
pmcid: 319460
Proietti E, Rossini S, Grohmann U, Mondanelli G (2020) Polyamines and kynurenines at the intersection of immune modulation. Trends Immunol 41(11):1037–1050
pubmed: 33055013
Prosser BL, Ward CW, Lederer WJ (2011) X-ROS signaling: rapid mechano-chemo transduction in heart. Science 333(6048):1440–1445
pubmed: 21903813
Rajakumar S, Abhishek A, Selvam GS, Nachiappan V (2020) Effect of cadmium on essential metals and their impact on lipid metabolism in Saccharomyces cerevisiae. Cell Stress Chaperones 25(1):19–33
pubmed: 31823289
Satarug S, Garrett SH, Sens MA, Sens DA (2011) Cadmium, environmental exposure, and health outcomes. Cien Saude Colet 16:2587
pubmed: 21655733
pmcid: 5967636
Sichilongo K, Padiso T, Turner Q (2020) AMDIS-Metab R data manipulation for the geographical and floral differentiation of selected honeys from Zambia and Botswana based on volatile chemical compositions using SPME-GC-MS. Eur Food Res Technol 246(8):1679–1690
Stincone A, Prigione A, Cramer T, Wamelink MM, Campbell K, Cheung E, Olin-Sandoval V, Grüning NM, Krüger A, Tauqeer Alam M, Keller MA, Breitenbach M, Brindle KM, Rabinowitz JD, Ralser M (2015) The return of metabolism: biochemistry and physiology of the pentose phosphate pathway. Biol Rev Camb Philos Soc 90(3):927–963
pubmed: 25243985
Stoiber TL, Shafer MM, Armstrong DE (2010) Differential effects of copper and cadmium exposure on toxicity endpoints and gene expression in Chlamydomonas reinhardtii. Environ Toxicol Chem 29(1):191–200
pubmed: 20821435
Sugino T, Shirai T, Kajimoto Y, Kajimoto O (2008) L-ornithine supplementation attenuates physical fatigue in healthy volunteers by modulating lipid and amino acid metabolism. Nutr Res 28(11):738–743
pubmed: 19083482
Sundaram A, Grant CM (2014) Oxidant-specific regulation of protein synthesis in Candida albicans. Fungal Genet Biol 67:15–23
pubmed: 24699161
Vandenbrouck T, Jones OA, Dom N, Griffin JL, De Coen W (2010) Mixtures of similarly acting compounds in Daphnia magna: from gene to metabolite and beyond. Environ Int 36(3):254–268
pubmed: 20117838
Vareda JP, Valente AJM, Durães L (2019) Assessment of heavy metal pollution from anthropogenic activities and remediation strategies: a review. J Environ Manag 246:101–118
Wang J, Lin M, Xu M, Yang ST (2016) Anaerobic fermentation for production of carboxylic acids as bulk chemicals from renewable biomass. Adv Biochem Eng Biotechnol 156:323–361
pubmed: 26907554
Webb AL, Hughes KA, Grand MM, Lohan MC, Peck LS (2020) Sources of elevated heavy metal concentrations in sediments and benthic marine invertebrates of the western Antarctic Peninsula. Sci Total Environ 698:134268
pubmed: 31783446
Wolff EC, Park MH, Folk JE (1990) Cleavage of spermidine as the first step in deoxyhypusine synthesis. The role of NAD. J Biol Chem 265(9):4793–4799
pubmed: 2108161
Wu G (2009) Amino acids: metabolism, functions, and nutrition. Amino Acids 37(1):1–17
Wu X, Liu Y, Yin S, Xiao K, Xiong Q, Bian S, Liang S, Hou H, Hu J, Yang J (2020) Metabolomics revealing the response of rice (Oryza sativa L.) exposed to polystyrene microplastics. Environ Pollut 266(Pt 1):115159
pubmed: 32663678
Xu JZ, Yang HK, Zhang WG (2018a) NADPH metabolism: a survey of its theoretical characteristics and manipulation strategies in amino acid biosynthesis. Crit Rev Biotechnol 38(7):1061–1076
pubmed: 29480038
Xu P, Lai C, Zeng G, Huang D, Chen M, Song B, Peng X, Wan J, Hu L, Duan A, Tang W (2018b) Enhanced bioremediation of 4-nonylphenol and cadmium co-contaminated sediment by composting with Phanerochaete chrysosporium inocula. Bioresour Technol 250:625–634
pubmed: 29220806
Yang S, Lian G (2020) ROS and diseases: role in metabolism and energy supply. Mol Cell Biochem 467(1–2):1–12
pubmed: 31813106
Yu R, Yuan X, Zhao Y, Hu G, Tu X (2008) Heavy metal pollution in intertidal sediments from Quanzhou Bay, China. J Environ Sci (china) 20(6):664–669
Zhang X, Hua M, Song C, Chi Z (2012) Occurrence and diversity of marine yeasts in Antarctica environments. J Ocean Univ China 11(1):70–74
Zhao D, Juhasz AL, Luo J, Huang L, Luo XS, Li HB, Ma LQ (2017) Mineral dietary supplement to decrease cadmium relative bioavailability in rice based on a mouse bioassay. Environ Sci Technol 51(21):12123–12130
pubmed: 28960068