Multiple catechols in human plasma after drinking caffeinated or decaffeinated coffee.
Caffeine
Catecholamines
Catechols
Coffee
Decaffeinated
Dihydrocaffeic Acid
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554
Informations de publication
Date de publication:
15 Nov 2021
15 Nov 2021
Historique:
received:
26
07
2021
revised:
05
10
2021
accepted:
09
10
2021
pubmed:
4
11
2021
medline:
2
2
2022
entrez:
3
11
2021
Statut:
ppublish
Résumé
Coffee is one of the most frequently consumed beverages worldwide. Research on effects of coffee drinking has focused on caffeine; however, coffee contains myriad biochemicals that are chemically unrelated to caffeine, including 3,4-dihydroxyphenyl compounds (catechols) such as caffeic acid and dihydrocaffeic acid (DHCA). This prospective within-subjects study examined effects of drinking caffeinated or decaffeinated coffee on plasma free (unconjugated) catechols measured by liquid chromatography with series electrochemical detection (LCED) after batch alumina extraction. To confirm coffee-related chromatographic peaks represented catechols, plasma was incubated with catechol-O-methyltransferase and S-adenosylmethionine before the alumina extraction; reductions in peak heights would identify catechols. Ten healthy volunteers drank 2 cups each of caffeinated and decaffeinated coffee on separate days after fasting overnight. With subjects supine, blood was drawn through an intravenous catheter up to 240 min after coffee ingestion and the plasma assayed by alumina extraction followed by LCED. Within 15 min of drinking coffee of either type, >20 additional peaks were noted in chromatographs from the alumina eluates. Most of the coffee-related peaks corresponded to free catechols. Plasma levels of the catecholamines epinephrine and dopamine increased with both caffeinated and decaffeinated coffee. Levels of other endogenous catechols were unaffected. Plasma DHCA increased bi-phasically, in contrast with other coffee-related free catechols. Drinking coffee-whether caffeinated or decaffeinated-results in the rapid appearance of numerous free catechols in the plasma. These might affect the disposition of circulating catecholamines. The bi-phasic increase in plasma DHCA is consistent with production by gut bacteria.
Sections du résumé
BACKGROUND
BACKGROUND
Coffee is one of the most frequently consumed beverages worldwide. Research on effects of coffee drinking has focused on caffeine; however, coffee contains myriad biochemicals that are chemically unrelated to caffeine, including 3,4-dihydroxyphenyl compounds (catechols) such as caffeic acid and dihydrocaffeic acid (DHCA).
OBJECTIVE
OBJECTIVE
This prospective within-subjects study examined effects of drinking caffeinated or decaffeinated coffee on plasma free (unconjugated) catechols measured by liquid chromatography with series electrochemical detection (LCED) after batch alumina extraction. To confirm coffee-related chromatographic peaks represented catechols, plasma was incubated with catechol-O-methyltransferase and S-adenosylmethionine before the alumina extraction; reductions in peak heights would identify catechols.
METHODS
METHODS
Ten healthy volunteers drank 2 cups each of caffeinated and decaffeinated coffee on separate days after fasting overnight. With subjects supine, blood was drawn through an intravenous catheter up to 240 min after coffee ingestion and the plasma assayed by alumina extraction followed by LCED.
RESULTS
RESULTS
Within 15 min of drinking coffee of either type, >20 additional peaks were noted in chromatographs from the alumina eluates. Most of the coffee-related peaks corresponded to free catechols. Plasma levels of the catecholamines epinephrine and dopamine increased with both caffeinated and decaffeinated coffee. Levels of other endogenous catechols were unaffected. Plasma DHCA increased bi-phasically, in contrast with other coffee-related free catechols.
INTERPRETATION
CONCLUSIONS
Drinking coffee-whether caffeinated or decaffeinated-results in the rapid appearance of numerous free catechols in the plasma. These might affect the disposition of circulating catecholamines. The bi-phasic increase in plasma DHCA is consistent with production by gut bacteria.
Identifiants
pubmed: 34731744
pii: S1570-0232(21)00469-4
doi: 10.1016/j.jchromb.2021.122988
pmc: PMC8647204
mid: NIHMS1755188
pii:
doi:
Substances chimiques
Caffeic Acids
0
Catechols
0
Coffee
0
3,4-dihydroxyphenylpropionic acid
1078-61-1
Caffeine
3G6A5W338E
caffeic acid
U2S3A33KVM
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
122988Subventions
Organisme : Intramural NIH HHS
ID : ZIA NS003034
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA NS003125
Pays : United States
Informations de copyright
Published by Elsevier B.V.
Références
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Dec 1;877(31):3965-74
pubmed: 19879819
Molecules. 2021 Mar 10;26(6):
pubmed: 33802064
J Pharmacol Exp Ther. 1962 Dec;138:360-75
pubmed: 14013351
Arch Biochem Biophys. 2010 Sep 1;501(1):98-105
pubmed: 20226754
J Neurosci. 2010 Apr 21;30(16):5525-32
pubmed: 20410106
N Engl J Med. 1978 Jan 26;298(4):181-6
pubmed: 339084
J Parkinsons Dis. 2015;5(2):255-62
pubmed: 25882059
Mov Disord. 1992 Oct;7(4):339-44
pubmed: 1484528
Nutrients. 2020 Jul 23;12(8):
pubmed: 32717940
Life Sci. 1977 Sep 1;21(5):625-36
pubmed: 904439
Circulation. 2002 Dec 3;106(23):2935-40
pubmed: 12460875
Neurology. 2000 Nov 14;55(9):1350-8
pubmed: 11087780
Clin Chem. 1986 Nov;32(11):2030-3
pubmed: 3096593
J Clin Gastroenterol. 2012 Oct;46 Suppl:S95-9
pubmed: 22955368
Br J Clin Pharmacol. 1985 Feb;19(2):275-8
pubmed: 3857069
J Chromatogr A. 2011 Jul 22;1218(29):4678-88
pubmed: 21676405
Crit Rev Food Sci Nutr. 2020;60(5):760-779
pubmed: 30614247
Br J Nutr. 2015 Apr 28;113(8):1220-7
pubmed: 25809126
J Nutr. 2007 Oct;137(10):2196-201
pubmed: 17884997
J Agric Food Chem. 2003 Feb 26;51(5):1474-9
pubmed: 12590501
Clin Exp Pharmacol Physiol Suppl. 1999 Apr;26:S41-53
pubmed: 10386253
Mol Nutr Food Res. 2011 May;55 Suppl 1:S35-43
pubmed: 21240902
J Agric Food Chem. 2017 Feb 1;65(4):752-758
pubmed: 28142243
Mov Disord. 2007 Nov 15;22(15):2242-8
pubmed: 17712848
Molecules. 2015 Jun 30;20(7):11930-40
pubmed: 26133759
Ann Neurol. 2001 Jul;50(1):56-63
pubmed: 11456310
Eur J Clin Nutr. 2008 Jul;62(7):908-15
pubmed: 17522612
Br J Nutr. 2010 Dec;104(11):1635-40
pubmed: 20691128
Biofactors. 2013 Nov-Dec;39(6):623-32
pubmed: 23904092
Neurology. 2012 Aug 14;79(7):651-8
pubmed: 22855866
J Nutr. 2008 Dec;138(12):2309-15
pubmed: 19022950
Ann Neurol. 2002 Sep;52(3):276-84
pubmed: 12205639
Org Biomol Chem. 2010 Nov 21;8(22):5199-211
pubmed: 20842300
J Chromatogr B Biomed Appl. 1994 Mar 4;653(2):131-8
pubmed: 8205240
Life Sci. 1976 Dec 1;19(11):1673-85
pubmed: 1004129
Drug Metab Dispos. 2009 Aug;37(8):1749-58
pubmed: 19460943
Acta Med Scand. 1967 Apr;181(4):431-8
pubmed: 6023454