Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory system transit.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
05 11 2021
Historique:
received: 25 11 2020
accepted: 15 10 2021
entrez: 6 11 2021
pubmed: 7 11 2021
medline: 28 12 2021
Statut: epublish

Résumé

While transcriptome- and proteome-wide technologies to assess processes in protein biogenesis are now widely available, we still lack global approaches to assay post-ribosomal biogenesis events, in particular those occurring in the eukaryotic secretory system. We here develop a method, SECRiFY, to simultaneously assess the secretability of >10

Identifiants

pubmed: 34741024
doi: 10.1038/s41467-021-26720-y
pii: 10.1038/s41467-021-26720-y
pmc: PMC8571348
doi:

Substances chimiques

Proteome 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6414

Commentaires et corrections

Type : ErratumIn

Informations de copyright

© 2021. The Author(s).

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Auteurs

Morgane Boone (M)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium. morganeboone@gmail.com.
Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium. morganeboone@gmail.com.
Department of Biochemistry and Biophysics, UCSF, San Francisco, CA, USA. morganeboone@gmail.com.

Pathmanaban Ramasamy (P)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.
Structural Biology Brussels, VUB, Brussels, Belgium.
Structural Biology Research Center, VIB, Brussels, Belgium.
Interuniversity Institute of Bioinformatics in Brussels (IB)2, ULB-VUB, Brussels, Belgium.

Jasper Zuallaert (J)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
Center for Biotech Data Science, Ghent University Global Campus, Songdo, Incheon, South Korea.
IDLab, ELIS, UGent, Ghent, Belgium.

Robbin Bouwmeester (R)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Berre Van Moer (B)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.

Davy Maddelein (D)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Demet Turan (D)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Niels Hulstaert (N)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Hannah Eeckhaut (H)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.

Elien Vandermarliere (E)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Lennart Martens (L)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Sven Degroeve (S)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium.
Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Wesley De Neve (W)

Center for Biotech Data Science, Ghent University Global Campus, Songdo, Incheon, South Korea.
IDLab, ELIS, UGent, Ghent, Belgium.

Wim Vranken (W)

Structural Biology Brussels, VUB, Brussels, Belgium.
Structural Biology Research Center, VIB, Brussels, Belgium.
Interuniversity Institute of Bioinformatics in Brussels (IB)2, ULB-VUB, Brussels, Belgium.

Nico Callewaert (N)

Center for Medical Biotechnology, VIB, Zwijnaarde, Belgium. nico.callewaert@vib-ugent.be.
Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium. nico.callewaert@vib-ugent.be.

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Classifications MeSH