Biochemical characterization and mutational studies of a thermostable endonuclease III from Sulfolobus islandicus REY15A.

Archaeal Proteins / chemistry Biochemical Phenomena DNA Repair Endonucleases / chemistry Mutation Sulfolobus / enzymology
Endonuclease III Sulfolobus islandicus Thymine glycol

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Dec 2021
Historique:
received: 14 08 2021
revised: 18 10 2021
accepted: 19 10 2021
pubmed: 9 11 2021
medline: 21 12 2021
entrez: 8 11 2021
Statut: ppublish

Résumé

Endonuclease III (EndoIII), which is ubiquitous in bacteria, Archaea and eukaryotes, plays an important role in excising thymine glycol (Tg) from DNA. Herein, we present evidence that an EndoIII from the hyperthermophilic crenarchaeon Sulfolobus islandicus REY15A (Sis-EndoIII) is capable of removing Tg from DNA at high temperature. Biochemical data show that the optimal temperature and pH of Sis-EndoIII are ca.70 °C and ca.7.0-8.0, respectively. Furthermore, the recombinant Sis-EndoIII retains relative weak activity without a divalent metal ion, and displays maximum activity in the presence of Mg

Identifiants

DOI: 10.1016/j.ijbiomac.2021.10.143 PMID: 34743941
pubmed: 34743941
pii: S0141-8130(21)02300-X
doi: 10.1016/j.ijbiomac.2021.10.143
pii:
doi:

Substances chimiques

Archaeal Proteins 0
Endonucleases EC 3.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

856-865

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Likui Zhang (L)

College of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, China; Guangling College, Yangzhou University, China. Electronic address: lkzhang@yzu.edu.cn.

Lei Wang (L)

College of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, China.

Leilei Wu (L)

College of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, China.

Donghao Jiang (D)

College of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, China.

Chengxuan Tang (C)

College of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, China.

Ying Wu (Y)

College of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, China.

Mai Wu (M)

College of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, China.

Min Chen (M)

College of Environmental Science and Engineering, Marine Science & Technology Institute, Yangzhou University, China. Electronic address: chenmin@yzu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'archée › Biochemical characterization and mutational...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Biochemical characterization and mutational...
questionsmedicales.fr › Phénomènes génétiques › Réparation de l'ADN › Biochemical characterization and mutational...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Endonucleases › Biochemical characterization and mutational...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Biochemical characterization and mutational...
questionsmedicales.fr › Archéobactéries › Crenarchaeota › Sulfolobale › Sulfolobaceae › Sulfolobus › Biochemical characterization and mutational...