Preconditioning of bull semen with sub-lethal oxidative stress before cryopreservation: Possible mechanism of mitochondrial uncoupling protein 2.
Bull semen cryopreservation
Hydrogen peroxide
Mitochondrial uncoupling protein 2
Nitric oxide
Sub-lethal stress
Journal
Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
received:
25
08
2021
revised:
30
10
2021
accepted:
02
11
2021
pubmed:
9
11
2021
medline:
23
4
2022
entrez:
8
11
2021
Statut:
ppublish
Résumé
Preconditioning of sperm using sub-lethal oxidative stress before cryopreservation is an innovative approach that can improve sperm cryo-survival. Mitochondrial uncoupling proteins (UCPs) are critical in reducing ROS level during stress conditions. The aim of the current study was to investigate whether mild sub-lethal stress induced by low concentrations of nitric oxide and hydrogen peroxide has a protective effect on quality parameters of post-thaw bull semen through modulations of mitochondrial uncoupling protein 2 (UCP2) expression. Semen samples were collected from 6 mature Holstein bulls, then mixed and divided into 8 aliquots: fresh, frozen control and frozen groups treated with NO: 0.1 (NO-0.1), 1(NO-1), 10 μM (NO-10), and H
Identifiants
pubmed: 34748771
pii: S0011-2240(21)00176-0
doi: 10.1016/j.cryobiol.2021.11.001
pii:
doi:
Substances chimiques
Uncoupling Protein 2
0
Nitric Oxide
31C4KY9ESH
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
63-69Informations de copyright
Copyright © 2021. Published by Elsevier Inc.