LC-MS/MS ceramides human white adipose tissue lipid identification lipid metabolism lipidomics obesity plasmalogens semi-absolute lipid quantification sphingolipids subcutaneous white adipose tissue triacylglycerols visceral white adipose tissue

Journal

Cell reports. Medicine
ISSN: 2666-3791
Titre abrégé: Cell Rep Med
Pays: United States
ID NLM: 101766894

Informations de publication

Date de publication:
19 10 2021
Historique:
received: 11 03 2021
revised: 29 07 2021
accepted: 26 08 2021
entrez: 10 11 2021
pubmed: 11 11 2021
medline: 11 11 2021
Statut: epublish

Résumé

Obesity, characterized by expansion and metabolic dysregulation of white adipose tissue (WAT), has reached pandemic proportions and acts as a primer for a wide range of metabolic disorders. Remodeling of WAT lipidome in obesity and associated comorbidities can explain disease etiology and provide valuable diagnostic and prognostic markers. To support understanding of WAT lipidome remodeling at the molecular level, we provide in-depth lipidomics profiling of human subcutaneous and visceral WAT of lean and obese individuals. We generate a human WAT reference lipidome by performing tissue-tailored preanalytical and analytical workflows, which allow accurate identification and semi-absolute quantification of 1,636 and 737 lipid molecular species, respectively. Deep lipidomic profiling allows identification of main lipid (sub)classes undergoing depot-/phenotype-specific remodeling. Previously unanticipated diversity of WAT ceramides is now uncovered.

Identifiants

pubmed: 34755127
doi: 10.1016/j.xcrm.2021.100407
pii: S2666-3791(21)00265-2
pmc: PMC8561168
doi:

Substances chimiques

Ceramides 0
Ethanolamines 0
Fatty Acids, Unsaturated 0
Lipids 0
Plasmalogens 0
Triglycerides 0
sphingadienine 25696-03-1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

100407

Commentaires et corrections

Type : CommentIn

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

M.B. received honoraria as a consultant and speaker from Amgen, AstraZeneca, Bayer, Boehringer-Ingelheim, Lilly, Novo Nordisk, Novartis, and Sanofi. All other authors declare no competing interests.

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Auteurs

Mike Lange (M)

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, Leipzig, Germany.
Center for Biotechnology and Biomedicine, University of Leipzig, Leipzig, Germany.

Georgia Angelidou (G)

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, Leipzig, Germany.
Center for Biotechnology and Biomedicine, University of Leipzig, Leipzig, Germany.

Zhixu Ni (Z)

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, Leipzig, Germany.
Center for Biotechnology and Biomedicine, University of Leipzig, Leipzig, Germany.

Angela Criscuolo (A)

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, Leipzig, Germany.
Center for Biotechnology and Biomedicine, University of Leipzig, Leipzig, Germany.
Thermo Fisher Scientific, Dreieich, Germany.

Jürgen Schiller (J)

Institute of Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Leipzig, Germany.

Matthias Blüher (M)

Medical Department III (Endocrinology, Nephrology and Rheumatology), University of Leipzig, Leipzig, Germany.
Helmholtz Institute for Metabolic, Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum München at the University of Leipzig and University Hospital Leipzig, Leipzig, Germany.

Maria Fedorova (M)

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, Leipzig, Germany.
Center for Biotechnology and Biomedicine, University of Leipzig, Leipzig, Germany.

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Classifications MeSH