A Multifunctional Neutralizing Antibody-Conjugated Nanoparticle Inhibits and Inactivates SARS-CoV-2.
Angiotensin-Converting Enzyme 2
/ physiology
Animals
Antibodies, Neutralizing
/ administration & dosage
Antibodies, Viral
/ administration & dosage
Antigen-Antibody Reactions
COVID-19
/ immunology
Drug Evaluation, Preclinical
Hot Temperature
Humans
Immunoconjugates
/ administration & dosage
Light
Mice
Nanoparticles
/ therapeutic use
Phosphatidylethanolamines
Polyethylene Glycols
Polymers
Receptors, Virus
/ physiology
SARS-CoV-2
/ immunology
Semiconductors
Spike Glycoprotein, Coronavirus
/ immunology
Thiadiazoles
Virus Inactivation
COVID-19
SARS-CoV-2
multifunctional nanoparticle
photothermal therapy
virus inactivation
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
revised:
05
10
2021
received:
29
07
2021
pubmed:
12
11
2021
medline:
27
1
2022
entrez:
11
11
2021
Statut:
ppublish
Résumé
The outbreak of 2019 coronavirus disease (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in a global pandemic. Despite intensive research, the current treatment options show limited curative efficacies. Here the authors report a strategy incorporating neutralizing antibodies conjugated to the surface of a photothermal nanoparticle (NP) to capture and inactivate SARS-CoV-2. The NP is comprised of a semiconducting polymer core and a biocompatible polyethylene glycol surface decorated with high-affinity neutralizing antibodies. The multifunctional NP efficiently captures SARS-CoV-2 pseudovirions and completely blocks viral infection to host cells in vitro through the surface neutralizing antibodies. In addition to virus capture and blocking function, the NP also possesses photothermal function to generate heat following irradiation for inactivation of virus. Importantly, the NPs described herein significantly outperform neutralizing antibodies at treating authentic SARS-CoV-2 infection in vivo. This multifunctional NP provides a flexible platform that can be readily adapted to other SARS-CoV-2 antibodies and extended to novel therapeutic proteins, thus it is expected to provide a broad range of protection against original SARS-CoV-2 and its variants.
Identifiants
pubmed: 34761549
doi: 10.1002/advs.202103240
pmc: PMC8646742
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Immunoconjugates
0
Phosphatidylethanolamines
0
Polymers
0
Receptors, Virus
0
Spike Glycoprotein, Coronavirus
0
Thiadiazoles
0
poly(2,6-(4,4-bis(2-ethylhexyl)-4H-cyclopenta(2,1-b-3,4-b0)dithiophene))-alt-4,7-(2,1,3-benzothiadiazole)
0
spike protein, SARS-CoV-2
0
1,2-distearoylphosphatidylethanolamine
1G4B5265CQ
Polyethylene Glycols
3WJQ0SDW1A
ACE2 protein, human
EC 3.4.17.23
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2103240Subventions
Organisme : NIDDK NIH HHS
ID : RC2DK122394
Pays : United States
Organisme : NIDA NIH HHS
ID : DP2 DA051912
Pays : United States
Organisme : NIH HHS
ID : DP2AI144245
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA009594
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007074
Pays : United States
Organisme : NIH HHS
ID : T32DK007074
Pays : United States
Organisme : NIAID NIH HHS
ID : DP2 AI144245
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2021 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Br J Surg. 2020 Jun;107(7):785-787
pubmed: 32191340
N Engl J Med. 2021 May 13;384(19):1866-1868
pubmed: 33761203
Nat Rev Immunol. 2020 Jun;20(6):355-362
pubmed: 32376901
J Photochem Photobiol B. 2010 Dec 2;101(3):271-8
pubmed: 20728373
N Engl J Med. 2020 May 21;382(21):1969-1973
pubmed: 32227757
Chem Soc Rev. 2019 Apr 1;48(7):2053-2108
pubmed: 30259015
Acta Pharm. 2013 Sep;63(3):335-58
pubmed: 24152895
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
View (Beijing). 2021 Jun;2(3):20200186
pubmed: 34766162
Bioconjug Chem. 2017 Apr 19;28(4):1115-1122
pubmed: 28177606
Cell Death Differ. 2021 Feb;28(2):626-639
pubmed: 33479399
Nature. 2020 Aug;584(7819):120-124
pubmed: 32454512
Adv Sci (Weinh). 2022 Jan;9(2):e2103240
pubmed: 34761549
Photomed Laser Surg. 2012 Sep;30(9):551-8
pubmed: 22853435
Cell. 2020 Jul 9;182(1):73-84.e16
pubmed: 32425270
Nat Biomed Eng. 2018 Jul;2(7):508-521
pubmed: 30906646
Nanomaterials (Basel). 2020 Jun 06;10(6):
pubmed: 32517253
ACS Nano. 2020 Jun 23;14(6):7617-7627
pubmed: 32437124
ACS Nano. 2020 May 26;14(5):5268-5277
pubmed: 32281785
Biomaterials. 2016 Nov;106:119-33
pubmed: 27561883
Semin Cutan Med Surg. 2013 Mar;32(1):41-52
pubmed: 24049929
Pharmaceutics. 2014 Jun 20;6(2):333-53
pubmed: 24955820
Science. 2020 Aug 7;369(6504):650-655
pubmed: 32571838
Nature. 2020 Aug;584(7821):353-363
pubmed: 32659783
Hum Vaccin Immunother. 2018 Jan 2;14(1):199-208
pubmed: 28933644
Nature. 2020 Aug;584(7821):450-456
pubmed: 32698192
Materials (Basel). 2019 Mar 07;12(5):
pubmed: 30866416
Nat Mater. 2016 Sep;15(9):1023-30
pubmed: 27348576
MAbs. 2019 Feb/Mar;11(2):297-304
pubmed: 30714473
Acta Biomater. 2019 Nov;99:307-319
pubmed: 31513911
Adv Mater. 2020 Oct;32(40):e2004452
pubmed: 32875709
Lancet. 2020 May 16;395(10236):1569-1578
pubmed: 32423584
J Control Release. 2020 Dec 10;328:251-262
pubmed: 32889053
Biochem Biophys Res Commun. 2014 Aug 22;451(2):208-14
pubmed: 25073113
Nat Protoc. 2019 May;14(5):1339-1376
pubmed: 30980031
Matter. 2021 Jun 2;4(6):2059-2082
pubmed: 33907732
ACS Nano. 2020 Sep 22;14(9):12125-12132
pubmed: 32808759
ACS Nano. 2020 Apr 28;14(4):5135-5142
pubmed: 32293168
J Control Release. 2014 Aug 10;187:91-100
pubmed: 24845126
N Engl J Med. 2020 Apr 30;382(18):e41
pubmed: 32212516
JAMA. 2020 Apr 28;323(16):1582-1589
pubmed: 32219428
Intensive Care Med. 2020 Apr;46(4):586-590
pubmed: 32125455
Am J Physiol Lung Cell Mol Physiol. 2004 May;286(5):L1002-8
pubmed: 14695119
J Gen Virol. 2020 Sep;101(9):925-940
pubmed: 32568027
Nature. 2020 May;581(7807):221-224
pubmed: 32225175
Nanoscale. 2017 Aug 17;9(32):11605-11618
pubmed: 28770914
Science. 2020 Aug 21;369(6506):956-963
pubmed: 32540903
Talanta. 2021 Jan 1;221:121435
pubmed: 33076064
J Thorac Dis. 2020 Jul;12(7):3611-3621
pubmed: 32802440
Cell Mol Immunol. 2020 Jun;17(6):647-649
pubmed: 32313207
Biointerphases. 2017 Mar 16;12(2):02D301
pubmed: 28301944
Science. 2020 Jun 12;368(6496):1274-1278
pubmed: 32404477
Clin Colorectal Cancer. 2019 Jun;18(2):e200-e209
pubmed: 30852125
Rev Med Virol. 2020 Sep;30(5):e2115
pubmed: 32614102
PLoS One. 2015 Nov 16;10(11):e0142816
pubmed: 26569396
J R Soc Interface. 2014 Dec 6;11(101):20140950
pubmed: 25401184
Cell Rep. 2021 May 4;35(5):109055
pubmed: 33905739
Lasers Med Sci. 2017 Nov;32(8):1825-1834
pubmed: 28712048
Plasmonics. 2021;16(4):1029-1033
pubmed: 33495692
Nat Rev Immunol. 2020 Jun;20(6):339-341
pubmed: 32317716
Biophys Rev. 2018 Apr;10(2):255-258
pubmed: 29256117
Cell. 2021 Jun 24;184(13):3452-3466.e18
pubmed: 34139176
J Aerosol Med. 2003 Summer;16(2):153-66
pubmed: 12823909
Science. 2020 Mar 27;367(6485):1444-1448
pubmed: 32132184
ACS Nano. 2020 Jun 23;14(6):6383-6406
pubmed: 32519842
Nature. 2010 Apr 8;464(7290):932-6
pubmed: 20357766
Emerg Microbes Infect. 2020 Dec;9(1):680-686
pubmed: 32207377
Front Immunol. 2018 Feb 05;9:155
pubmed: 29459867
Nat Commun. 2020 Jul 9;11(1):3523
pubmed: 32647131
Front Cell Infect Microbiol. 2018 Feb 15;8:44
pubmed: 29497604
J Control Release. 2014 Dec 28;196:344-54
pubmed: 25451545
EClinicalMedicine. 2020 May 01;23:100370
pubmed: 32632410
Antiviral Res. 2016 Jan;125:1-7
pubmed: 26585243
Proc Natl Acad Sci U S A. 2007 May 29;104(22):9422-7
pubmed: 17517625
N Engl J Med. 2020 Aug 6;383(6):585-586
pubmed: 32492298
Nat Med. 2020 Jul;26(7):1037-1040
pubmed: 32393804
Nat Commun. 2019 Sep 24;10(1):4336
pubmed: 31551496
Lancet. 2020 Mar 28;395(10229):1033-1034
pubmed: 32192578
Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):10149-54
pubmed: 15220475
Science. 2020 Aug 7;369(6504):643-650
pubmed: 32540902
JAMA. 2021 Apr 6;325(13):1261-1262
pubmed: 33571363
Br J Cancer. 2020 Sep;123(5):694-697
pubmed: 32572174
Nat Biotechnol. 2020 Jul;38(7):789-791
pubmed: 32504046
Nature. 2020 May;581(7807):215-220
pubmed: 32225176
Lasers Med Sci. 2018 Sep;33(7):1431-1439
pubmed: 30006754
Nat Rev Cardiol. 2020 May;17(5):259-260
pubmed: 32139904
Nano Lett. 2020 Jul 8;20(7):5570-5574
pubmed: 32551679
J Virol. 2003 Aug;77(15):8322-8
pubmed: 12857901
Cell. 2020 May 14;181(4):894-904.e9
pubmed: 32275855
J Virol. 2020 Feb 14;94(5):
pubmed: 31826992
J Photochem Photobiol B. 2020 Jan;203:111731
pubmed: 31935633
Sci Rep. 2017 Oct 4;7(1):12670
pubmed: 28978926
AAPS J. 2015 Mar;17(2):339-51
pubmed: 25604608