An optimized 3D-printed perfusion bioreactor for homogeneous cell seeding in bone substitute scaffolds for future chairside applications.
Animals
Biomarkers
Bioreactors
Bone Regeneration
Bone Substitutes
Cell Culture Techniques
Cell Differentiation
Cells, Cultured
Equipment Design
Humans
Immunohistochemistry
Mesenchymal Stem Cells
/ cytology
Osteogenesis
Perfusion
Positron Emission Tomography Computed Tomography
Printing, Three-Dimensional
Tissue Engineering
/ methods
Tissue Scaffolds
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 11 2021
15 11 2021
Historique:
received:
04
03
2021
accepted:
20
10
2021
entrez:
16
11
2021
pubmed:
17
11
2021
medline:
1
2
2022
Statut:
epublish
Résumé
A clinical implementation of cell-based bone regeneration in combination with scaffold materials requires the development of efficient, controlled and reproducible seeding procedures and a tailor-made bioreactor design. A perfusion system for efficient, homogeneous, and rapid seeding with human adipogenic stem cells in bone substitute scaffolds was designed. Variants concerning medium inlet and outlet port geometry, i.e. cylindrical or conical diffuser, cell concentration, perfusion mode and perfusion rates were simulated in silico. Cell distribution during perfusion was monitored by dynamic [
Identifiants
pubmed: 34782672
doi: 10.1038/s41598-021-01516-8
pii: 10.1038/s41598-021-01516-8
pmc: PMC8593024
doi:
Substances chimiques
Biomarkers
0
Bone Substitutes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
22228Subventions
Organisme : Ministry of Economy, Construction and Tourism of the state Mecklenburg-Vorpommern and the European Regional Development Fund within the project "MediStream"
ID : TBI-V-1-108-VBW-037
Informations de copyright
© 2021. The Author(s).
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