The cryo-EM structure of the chloroplast ClpP complex.

Chaperonins / ultrastructure Chlamydomonas reinhardtii / enzymology Chloroplasts / ultrastructure Cryoelectron Microscopy

Journal

Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677

Informations de publication

Date de publication:
11 2021
Historique:
received: 03 11 2020
accepted: 12 10 2021
entrez: 16 11 2021
pubmed: 17 11 2021
medline: 27 1 2022
Statut: ppublish

Résumé

Protein homoeostasis in plastids is strategically regulated by the protein quality control system involving multiple chaperones and proteases, among them the Clp protease. Here, we determined the structure of the chloroplast ClpP complex from Chlamydomonas reinhardtii by cryo-electron microscopy. ClpP contains two heptameric catalytic rings without any symmetry. The top ring contains one ClpR6, three ClpP4 and three ClpP5 subunits while the bottom ring is composed of three ClpP1

Identifiants

DOI: 10.1038/s41477-021-01020-x PMID: 34782772
pubmed: 34782772
doi: 10.1038/s41477-021-01020-x
pii: 10.1038/s41477-021-01020-x
doi:

Substances chimiques

Chaperonins EC 3.6.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1505-1515

Informations de copyright

© 2021. The Author(s), under exclusive licence to Springer Nature Limited.

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Auteurs

Ning Wang (N)

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

Yifan Wang (Y)

College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.
ORCID: 0000-0001-9966-682X

Qian Zhao (Q)

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.
ORCID: 0000-0001-6555-4467

Xiang Zhang (X)

State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.

Chao Peng (C)

National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai Advanced Research Institute, CAS, Shanghai, China.
ORCID: 0000-0003-0779-4037

Wenjuan Zhang (W)

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

Yanan Liu (Y)

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

Olivier Vallon (O)

Institut de Biologie Physico-Chimique, Sorbonne Université, Paris, France.

Michael Schroda (M)

Molecular Biotechnology & Systems Biology, TU Kaiserslautern, Kaiserslautern, Germany.

Yao Cong (Y)

State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China. cong@sibcb.ac.cn.
Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China. cong@sibcb.ac.cn.
ORCID: 0000-0002-7164-8694

Cuimin Liu (C)

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China. cmliu@genetics.ac.cn.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China. cmliu@genetics.ac.cn.
ORCID: 0000-0003-4982-4804

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Chaperons moléculaires › Protéines du choc thermique › Chaperonines › The cryo-EM structure of the chloroplast ClpP complex.
questionsmedicales.fr › Eucaryotes › Viridiplantae › Chlorophyta › Chlamydomonas › Chlamydomonas reinhardtii › The cryo-EM structure of the chloroplast ClpP complex.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Plastes › Chloroplastes › The cryo-EM structure of the chloroplast ClpP complex.
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › The cryo-EM structure of the chloroplast ClpP complex.