Production of Vaccines Using Biological Conjugation.

Campylobacter jejuni Escherichia coli / genetics Glycoconjugates Polysaccharides Vaccines, Conjugate
Biological conjugation PGCT PglB Streptococcus pneumoniae Vaccine

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 16 11 2021
pubmed: 17 11 2021
medline: 15 12 2021
Statut: ppublish

Résumé

The production of conjugate vaccines within an E. coli (Escherichia coli) host provides an inexhaustible supply without the need for culture of pathogenic organisms. The machinery for expression of glycan and acceptor protein, as well as the coupling enzyme, are all housed within the E. coli chassis, meaning that there are no additional steps required for individual purification and chemical conjugation of components. In addition, there are far fewer purification steps necessary to obtain a purified glycoconjugate for use in vaccine testing. Here we describe production and purification of a HIS-tagged Campylobacter jejuni AcrA protein conjugated to Streptococcus pneumoniae serotype 4 capsule.

Identifiants

DOI: 10.1007/978-1-0716-1900-1_15 PMID: 34784042
pubmed: 34784042
doi: 10.1007/978-1-0716-1900-1_15
doi:

Substances chimiques

Glycoconjugates 0
Polysaccharides 0
Vaccines, Conjugate 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

281-300

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Emily J Kay (EJ)

Faculty of Infectious and Tropical Diseases, Department of Infection Biology, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK.

Vanessa S Terra (VS)

Faculty of Infectious and Tropical Diseases, Department of Infection Biology, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK. Vanessa.Terra@lshtm.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter › Campylobacter jejuni › Production of Vaccines Using Biological Conjugation.
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Production of Vaccines Using Biological Conjugation.
questionsmedicales.fr › Glucides › Glycoconjugués › Production of Vaccines Using Biological Conjugation.
questionsmedicales.fr › Glucides › Polyosides › Production of Vaccines Using Biological Conjugation.
questionsmedicales.fr › Facteurs biologiques › Antigènes › Vaccins synthétiques › Vaccins conjugués › Production of Vaccines Using Biological Conjugation.